Publications 1998 – Molecular Membrane Biology

I. Albert, A. W. Rutherford, H. Grav, J. Kellermann, and H. Michel
The 18 kDa Cytochrome c553 from Heliobacterium gestii: Gene Sequence and Characterization of the Mature Protein.
Biochemistry, 37, 9001-9008 (1998).

J. Behr, P. Hellwig, W. Mäntele, and H. Michel
Redox Dependent Changes at the Heme Propionates in Cytochrome c Oxidase from Paracoccus denitrificans. Direct Evidence from FTIR Difference Spectroscopy in Combination with Heme Propionate 13C Labeling.
Biochemistry, 37, 7400-7406 (1998).

J. Behr, W. Haase, G. Maul, S. Vasudevan, and H. Reiländer
Effect of N-glycosylation on production of the rat m3 muscarinic acetylcholine receptor in baculovirus infected insect cells.
Biochem. Soc. Trans., 26, 704-709 (1998).

I.-P. Chen and H. Michel
Cloning, Sequencing, and Characterization of the recA Gene from Rhodopseudomonas viridis and Construction of a recA Strain.
J. Bacteriol., 180, 3227-3232, 1998.

I.-P. Chen and H. Michel
Site-directed mutagenesis of the cytochrome subunit in the photosynthetic reaction center from Rps. viridis.
In: Photosynthesis: Mechanisms and Effects, Vol. 1 (Garab, G., ed.) Kluwer Academic Publishers, Dordrecht, NL (in press:1998).

U. Ermler, W. Grabarse, S. Shima, M. Goubeaud, and R. K. Thauer
Mechanismus der mikrobiellen Methanbildung.
Biospektrum 4, 20-24 (1998).

U. Ermler, W. Grabarse, S. Shima, M. Goubeaud, and R. K. Thauer
Active sites of transition metal enzymes with focus on nickel.
Curr. Opinion in Struct. Biol. 8, 749-758 (1998).

G. Ollesch, A. Kaunzinger, D. Juchelka, M. Schubert-Zsilavecz, and U. Ermler
Phospholipid bound to the flavohemoprotein from Alcaligenes eutrophus.
(submitted: Eur. J. Biochem. 1998).

G. Fritzsch, L. Kampmann, G. Kapaun, and H. Michel
Water clusters in the reaction centre of Rhodobacter sphaeroides.
Photosynthesis Research, 55, 127-132, (1998).

G. Fritzsch
Obtaining Crystal Structures from Bacterial Photosynthetic Reaction Centers.
Methods Enzymol., 297, pp. 57-77 (1998).

G. Fritzsch and A. Kuglstatter
The Structure of Reaction Centers from Purple Bacteria.
(submitted 1998: Advances in Photosynthesis, Erscheinungsdatum1999).

P. K. Fyfe, K. E. McAuley-Hecht, J. P. Ridge, S. M. Prince, G. Fritzsch, N. W. Isaacs, R. J. Cogdell, and M. R. Jones
Crystallographic studies of mutant reaction centres from Rhodobacter sphaeroides.
Photosynthesis Research, 55, 133-140, (1998).

R. Gross, J. Simon, C. R. D. Lancaster, and A. Kröger
Identification of histidine residues in Wolinella succinogenes hydrogenase that are essential for menaquinone reduction by H2.
Mol. Microbiol., 30, 639-646 (1998).

W. Haase, H.M. Weiß and H. Reiländer
Localization of the heterologously expressed, myc-tagged 5HT5A receptor by post-embedding immunogold staining of ultrathin sections prepared from recombinant Pichia pastoris. (in press: In Pichia Protocols series: Methods in Molecular Biology (Higgins, D.R. and Gregg, J. M., eds.), Humana Press Inc.Totowa, NJ, pp. 241-247 (1998).

W. Hampe, R.-H. Voß, W. Haase, F. Boege, H. Reiländer and H. Michel
Expression in Escherichia coli and insect cells and purification of a proteolytically stable human ß2-adrenergic receptor/Maltose-binding protein fusion.
(submitted: 1998).

P. Hellwig, C. Ostermeier, B. Ludwig, H. Michel and W. Mäntele
Electrochemically Induced FTIR Difference Spectra of the Cytochrome c Oxidase from Paracoccus denitrificans.
(in press: PNAS book, 1998)

P. Hellwig, J. Behr, C. Ostermeier, O.-M. H. Richter, U. Pfitzner, A. Odenwald, B. Ludwig, H. Michel and W. Mäntele
Involvement of Glutamic Acid 278 in the Redox Reaction of the Cytochrome c Oxidase from Paracoccus denitrificans Investigated by FT-IR Spectroscopy.
Biochemistry, 37, 7390-7399 (1998).

A. Ivanovic, H. Reiländer, B. Laube, and J. Kuhse
Expression and Initial Characterization of a Soluble Glycine Binding Domain of the N-Methyl-D-aspartate Receptor NR1 Subunit.
J. Biol. Chem., 273, 19933-19937 (1998).

A. Kannt, C. R. D. Lancaster and H. Michel
The role of electrostatic interactions for cytochrome c oxidase function.
J. Bioenerg. Biomembr., 30, 81-87 (1998).

A. Kannt, H. Michel, M. R. Cheesman, A. J. Thomson, A. B. Dreusch, H. Körner, and W. G. Zumft: The Electron Transfer Centers of Nitric Oxide Reductase: Homology with the Heme-Copper Oxidase Family. In: Biological electron-transfer chains: Genetics, composition and mode of operation. (Canters, G. W. and Vijgenboom, E., eds). Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 279-291 (1998).

A. Kannt, C. R. D. Lancaster, and H. Michel
The Coupling of Electron Transfer and Proton Translocation: Electrostatic Calculations on Paracoccus denitrificans Cytochrome c Oxidase.
Biophys. J., 74, 708-721 (1998).

A. Kannt, T. Soulimane, G. Buse, A. Becker, E. Bamberg and H. Michel
Electrical current generation and proton pumping catalyzed by the ba3-type cytochrome c oxidase from Thermus thermophilus.
FEBS Lett. 434, 17-22 (1998).

A. Kuglstatter, G. Fritzsch, and H. Michel
Crystallographic Analysis of the Mutant Reaction Center M197 (FY) from Rhodobacter sphaeroides.
In: Photosynthesis: Mechanisms and Effects, Vol. 1 (Garab, G., ed.) Kluwer Academic Publishers, Dordrecht, NL (in press:1998).

C. R. D. Lancaster
Ubiquinone reduction and protonation in photosynthetic reaction centres from Rhodopseudomonas viridis: X-ray structure and their functional implications.
Biochim. Biophys. Acta., 1365, 143-150 (1998).

C. R. D. Lancaster
The structure of the Rhodopseudomonas viridis reaction centre- an overview and recent advances.
In: Photosynthesis: Mechanisms and Effects, Vol. 1 (Garab, G., ed.) Kluwer Academic Publishers, Dordrecht, NL (in press:1998).

C. R. D. Lancaster and H. Michel
Refined crystal structures of reaction centres from Rhodopseudomonas viridis in complexes with the herbicide atrazine and two chiral atrazine derivatives aslo lead to a new model of the bound carotenoid.
(submitted: J. Mol. Biol., Oct. 1998).

T. Lenhard, K. Marheineke, B. Lingen, W. Haase, R. Hammermann, H. Michel, and H. Reiländer
Characterization of the Human Dopamine Transporter Heterologously Expressed in BHK-21 Cells.
Cell. Mol. Neurobiol., 18, 347-360 (1998).

K. Marheineke, T. Lenhard, W. Haase, T. Beckers, H. Miche,l and H. Reiländer
Characterization of the human gonadotropin-releasing hormone receptor heterologously produced using the baculovirus/insect cell and the Semliki-Forest virus systems.
Cell. Mol. Neurobiol., 18, 509-523 (1998).

K. Marheineke, S. Grünewald, W. Christie, and H. Michel
Lipid composition of Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) insect cells used for baculovirus infection.
(in press: FEBS Lett., 1998).

H. Michel
Membrane Proteins at Work: Bacterial Photosynthetic Reaction Centers and Cytochrome c Oxidase.
In: 1997 Yakult International Symposium. Protein Structures at Work. Yakult Honsha Co., Ltd. Tokyo, Japan, pp. 1-8 (1998).

H. Michel, J. Behr, A. Harrenga and A. Kannt
Cytochrome c Oxidase: Structure and Spectroscopy.
Annu. Rev. Biophys. Biomol. Struct., 27, 329-356 (1998).

H. Michel
Kristallisation und Strukturaufklärung von Membranproteinen: Die Cytochrom-c-Oxidase (Warburgsches Atmungsferment) des Bodenbakteriums Paracoccus denitrificans.
In: Jahrbuch 1997 der Deutschen Akademie der Naturforscher Leopoldina (Halle/Saale), Leopoldina (R. 3) 43, 269-279 (1998).

H. Michel
The mechanism of proton pumping by cytochrome c oxidase.
Proc. Natl. Acad. Sci.. U.S.A., 95, 12819-12824 (1998)

H. Michel, S. Iwata, and C. Ostermeier
Crystallization, Structure and Possible Mechanism of Action of Cytochrome c Oxidase from the Soil Bacterium Paracoccus denitrificans.
(submitted: In: Frontiers in Cellular Bioenergetics. Kluwer Academic/Plenum Publishers, 1998).

H. Reiländer, C. Reinhart and A. Szmolenszky
Large scale expression of receptors in yeasts.
(submitted: CRC-Press, 1998).

H. Reiländer and H. M. Weiss
Production of G protein-coupled receptors in yeast.
Curr. Opin. Biotech., 9, 510-517 (1998).

G. Schmees, K. Höner zu Bentrup, E. Schneider, D. Vinzenz and U. Ermler
Crystallization and preliminary X-ray analysis ot the bacterial ATP-Binding-Cassette (ABC)-protein MalK.
(in press: Acta. Cryst. D 54, 1998)

J. Servos, H. Reiländer, and H. Zimmermann
Heterologus Expression and Purification of Catalytically Acitve Soluble Ecto-5’-Nucleotidase.
(in press: Drug Development Research, 1998).

S. Shima, C. Tziatzios, D. Schubert, H. Fukada, T. Katsutada, and U. Ermler
Lyotropic salt-induced changes in monomer/dimer/tetramer association equilibrium of formyltransferase from the hyperthermophilic Methanopyrus kandleri in relation to activity and thermostability of the enzyme.
Eur. J. Biochem. 285, 85-92 (1998.

J. Tandori, P. Sebban, H. Michel, and L. Baciou
Proton uptake and electron transfer in reaction centers from Rb. sphaeroides modified in the proximity of a water chain.
In: Photosynthesis: Mechanisms and Effects, Vol. 1 (Garab, G., ed.) Kluwer Academic Publishers, Dordrecht, NL (in press:1998).

H. M. Weiss, W. Haase, H. Michel, and H. Reiländer
Comparative biochemical and pharmacological characterization of the mouse 5HT5A 5-hydroxytryptamine receptor and the human ß2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris.
Biochem. J., 330, 1137-1147 (1998).

H. M. Weiss, W. Haase, and H. Reiländer
Expression of an Integral Membrane Protein, the 5HT5A Receptor.
In: Pichia Protocols series: Methods in Molecular Biology (Higgins, D.R. and Gregg, J. M., eds.), Humana Press Inc., Totowa, NJ., 103, pp. 227-239 (1998).

H. M. Weiss and H. Reiländer
Das Pichia pastoris Expressionssystem.
In: Gentechnische Methoden (Gassen, H. G. and Schrimpf, G., eds.)
(in press: Gustav-Fischer Verlag, 1998).

M. Wendling, F. van Mourik, I. H. M. van Stokkum, J. M. Salverda, H. Michel and R. van Grondelle
Low-Intensity Pump-Probe Measurements on the B800 Band of Rhodospirillum molischianum.
In: Photosynthesis: Mechanisms and Effects, Vol. 1 (Garab, G., ed.) Kluwer Academic Publishers, Dordrecht, NL (in press:1998).


Ph. D. Theses

Markus Weiß: Produktion von G-Protein-gekoppelten Rezeptoren in der methylotrophen Hefe Pichia pastoris und deren Charakterisierung und Reinigung. Frankfurt 1998.

Ralf-Holger Voß: Charakterisierung gentechnisch veränderter ß2-adrenerger Rezeptoren. Frankfurt 1998.


Diploma Theses

Guido Kapaun: Röntgenkristallographische Untersuchungen am Reaktionszentrum von Rhodobacter sphaeroides: hochaufgelöste Wildtypstruktur und Cofaktor-Austausch. Frankfurt 1998.

Clemens Grimm: Die Kristallstruktur der Formylmethanofuran: Tetrahydromethanopterin-Formyltransferase aus Methanosarcina barkeri. Frankfurt 1998.

Leander Grode: Isolierung und Charakterisierung der H+/K+-ATPase aus Schweinemägen. Frankfurt 1998.

 

 

Contact:

Max Planck Institute of Biophysics

Prof. Dr. Dr. h.c. Hartmut Michel, Managing Director
Department of Molecular Membrane Biology
Secretary: S. McCormack

Phone: +49 (0) 69 6303-1001
Fax: +49 (0) 69 6303-1002

E-mail: secretariat.michel(at)biophys.mpg.de