Acharya, P., E. Warkentin, U. Ermler, R.K. Thauer and S. Shima: The structure of a formyl-methanofuran: tetrahydromethanopterin formyltransferase in complex with its coenzymes. J. Mol. Biol. 357, 870-879 (2006).

André, N., N. Cherouati, C. Prual, T. Steffan, G. Zeder-Lutz, T. Magnin, F. Pattus, H. Michel, R. Wagner and C. Reinhart: Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Sci. 15, 1115-1126 (2006).

Dierl, S., C. Reinhart and H. Reiländer: Das Pichia pastoris Expressionssystem, in: “Gentechnische Methoden”, Eine Sammlung von Arbeitsanleitungen für das molekularbiologische Labor. Jahnson, Monika (Hrsg.) Elsevier/Spektrum Verlag, München, 2006.

Farver, O., E. Grell, B. Ludwig, H. Michel and I. Pecht: Rates and Equilibrium of Cu_A to Heme a Electron Transfer in Paracoccus denitrificans Cytochrome c Oxidase. Biophys. J. 90, 2131-2137 (2006).

Hagemeier, C.H., M. Krüer, R.K. Thauer, E. Warkentin and U. Ermler: Insight into the mechanism of biological methanol activation based on the crystal structure of the methanol-cobalamin methyltransferase complex. Proc. Natl. Acad. Sci. U.S.A. 103, 18917-18922 (2006).

Hassaine, G., R. Wagner, J. Kempf, N. Hassaine, C. Prual, N. André, C. Reinhart and K. Lundstrom: Semliki Forest Virus vectors for overexpression of 101 G Protein-coupled receptors in mammalian host cells. Protein Expression Purif. 45, 343-351 (2006).

Herzog, E., T. Frigato, V. Helms and C.R.D. Lancaster: Energy barriers of proton transfer reactions between amino acid side chain analogs and water from ab initio calculations. J. Comput. Chem. 27, 1534-1547 (2006).

Hielscher R., T. Wenz, S. Stolpe, C. Hunte, T. Friedrich and P. Hellwig: Monitoring redox-dependent contribution of lipids in Fourier tranform infrared difference spectra of complex I from Escherichia coli. Biopolymers 82, 291-294 (2006).

Lancaster, C.R.D., A.H. Haas, M.G. Madej and M. Mileni: Recent progress on obtaining theoretical and experimental support for the “E-pathway hypothesis” of coupled transmembrane electron and proton transfer in dihaem-containing quinol:fumarate reductase. (Special Issue: Proton Transfer Reactions) Biochim. Biophys. Acta, Bioenerg. 1757, 988-995 (2006).

Lundstrom K., R. Wagner, C. Reinhart, A. Desmyter, N. Cherouati, T. Magnin, G. Zeder-Lutz, M. Courtot, C. Prual, N. Andre, G. Hassaine, H. Michel, C. Cambillau and F. Pattus: Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems. J. Struct. Funct. Genomics 7, 77-91 (2006).

Ma, X., S. Panjikar, J. Koepke, E. Loris and J. Stöckigt: The Structure of Rauvolfia serpentina Strictosidine Synthase Is a Novel Six-Bladed ß-Propeller Fold in Plant Proteins. The Plant Cell 18, 907-920 (2006).

MacMillan, F., K. Budiman, H. Angerer and H. Michel: The role of tryptophan 272 in the Paracoccus denitrificans cytochrome c oxidase. FEBS Lett. 580, 1345-1349 (2006).

Madej, M.G., H.R. Nasiri, N.S. Hilgendorff, H. Schwalbe and C.R.D. Lancaster: Evidence for transmembrane proton transfer in a dihaem-containing membrane protein complex. EMBO J. 25, 4963-4970 (2006).

Madej, M.G., H.R. Nasiri, N.S. Hilgendorff, H. Schwalbe, G. Unden and C.R.D. Lancaster: Experimental evidence for proton-motive force-dependent catalysis by the diheme-containing succinate:menaquinone oxidoreductase from the Gram-positive bacterium Bacillus licheniformis. Biochemistry 45, 15049-15055 (2006).

Mileni, M., F. MacMillan, C. Tziatzios, K. Zwicker, A.H. Haas, W. Mäntele, J. Simon and C.R.D. Lancaster: Heterologous production in Wolinella succinogenes and characterisation of the quinol:fumarate reductase enzymes from Helicobacter pylori and Campylobacter jejuni. Biochem J. 395, 191-201 (2006).

Nasiri, H.R., M.G. Madej, C.R.D. Lancaster, H. Schwalbe and M. Bolte: 2-Hydroxy-2-3-(3-oxobutyl)naphtalene-1,4-dione. Acta Crystallogr., Sect C 62, 0671-0673 (2006).

Olkhova, E., C. Hunte, E. Screpanti, E. Padan and H. Michel: Multiconformation continuum electrostatics analysis of the NhaA  Na⁺/H⁺ antiporter of  Escherichia coli with functional implications. Proc. Natl. Acad. Sci. U.S.A. 103, 2629-2634 (2006).

Peng, G., M. Bostina, M. Radermacher, I. Rais, M. Karas and H. Michel: Biochemical and electron microscopic characterization of the F₁F₀ ATP Synthase from the hyperthermophilic eubacterium Aquifex aeolicus. FEBS Lett. 580, 5934-5940 (2006).

Pilak, O., B. Mamat, S. Vogt, C.H. Hagemeier, R.K. Thauer, S. Shima, C. Vonrhein, E. Warkentin and U. Ermler: The crystal structure of the apoenzyme of the iron-sulphur cluster-free hydrogenase. J. Mol. Biol. 358, 798-809 (2006).

Reinhart, C. and C. Krettler: Expression of membrane proteins in yeasts, in: “Structural Genomics on Membrane Proteins”. Lundstrom, Kenneth (Editor), CRC Press/Taylor & Francis, Boca Raton, FL, USA, 2006.

Schiffer A., G. Fritz, P.M.H. Kroneck and U. Ermler: Reaction mechanism of the iron-sulfur flavoenzyme adenosine-5 ‘-phosphosulfate reductase based on the structural characterization of different enzymatic states. Biochemistry 45, 2960-2967 (2006).

Screpanti, E., E. Padan, A. Rimon, H. Michel and C. Hunte: Crucial steps in the structure determination of the Na⁺/H⁺ antiporter NhaA in its native conformation. J. Mol. Biol. 362, 192-202 (2006).

Shukla, A.K., W. Haase, C. Reinhart and H. Michel: Biochemical and pharmacological characterization of the human bradykinin subtype 2 receptor produced in mammalian cells using the Semliki Forest virus system. Biol. Chem. 387, 569-576 (2006).

Shukla, A.K., W. Haase, C. Reinhart and H. Michel: Functional overexpression and characterization of human bradykinin subtype 2 receptor in insect cells using the baculovirus system. J. Cell. Biochem 99, 868-877 (2006).

Shukla, A.K., C. Reinhart and H. Michel: Dimethylsulphoxide as a tool to increase functional expression of heterologously produced GPCRs in mammalian cells. FEBS Lett. 580, 4261-4265 (2006).

Shukla, A.K., C. Reinhart and H. Michel: Comparative analysis of the human angiotensin II type 1a receptor heterologously produced in insect cells and mammalian cells. Biochem. Biophys. Res. Commun. 349, 6-14 (2006).

Surade, S., M. Klein, P.C. Stolt-Bergner, C. Münke, A. Roy and H. Michel: Comparative analysis and “expression space” coverage of the production of prokaryotic membrane proteins for structural genomics. Protein Sci. 15, 2178-2189 (2006).

Wenz, T., P. Hellwig, F. MacMillan, B. Meunier and C. Hunte: Probing the role of E272 in quinol oxidation of mitochondrial complex III. Biochemistry 45, 9042-9052 (2006).

Zeder-Lutz, G., N. Cherouati, C. Reinhart, F. Pattus and R. Wagner: Dot-blot immunodetection as a versatile and high-throughput assay to evaluate recombinant GPCRs produced in the yeast Pichia pastoris. Protein Expression and Purification 50, 118-127 (2006).


Ph.D. Theses

André, N.: Enhancement of the Production of Functional G Protein-Coupled Receptors in the Yeast Pichia pastoris and Purification of the Rat Neurokinin 2 Receptor. Frankfurt 2006.

Huo, D.: Heterologe Expression des humanen ß₂-adrenergen Rezeptors im Semliki Forest Virus-Expressionssystem. Frankfurt 2006.

Prual, C.: Production of the human adonosine A_2A receptor in Pichia pastoris, its solubilization and purification, and the selection of a specific single-chain Fv fragment by phage display. Frankfurt 2006.

Screpanti, E.: Structure of NhaA, a Na⁺/H⁺ antiporter from Escherichia coli and insights into mechanism of action and regulation by pH. Frankfurt 2006.

Shukla, A.K.: Heterologous Production, Characterization and Isolation of Selected G Protein-Coupled Receptors for Structural Studies. Frankfurt 2006.

Solmaz, S.: The Interacting of the Cytochrome bc₁ Complex with its Substrate Cytochrome c: High Resolution Structure and Implications for Transient Binding. Frankfurt 2006.


Diploma Theses

Lößner, C.: Isolierung und Charakterisierung von rekombinanten NCS -Transportern in Escherichia coli. Mainz 2006 Müller, F.: Produktion der C4-Dicarboxylat-Transporter DcuA, DcuB, DcuC und “Transporter” aus Salmonella typhimurium in Escherichia coli. Frankfurt 2006.