Hartmut Michel: Molecular Membrane Biology – Department Publications

Journal Article (26)

2006
Journal Article
Hagemeier, C. H.; Krüer, M.; Thauer, R.; Warkentin, E.; Ermler, U.: Insight into the mechanism of biological methanol activation based on the crystal structure of the methanol-cobalamin methyltransferase complex. Proceedings of the National Academy of Sciences of the United States of America 103 (50), pp. 18917 - 18922 (2006)
Journal Article
Madej, M. G.; Nasiri, H. R.; Hilgendorff, N. S.; Schwalbe, H.; Unden, G.; Lancaster, C. R. D.: Experimental Evidence for Proton Motive Force-Dependent Catalysis by the Diheme-Containing Succinate:Menaquinone Oxidoreductase from the Gram-Positive Bacterium Bacillus licheniformis. Biochemistry 45 (50), pp. 15049 - 15055 (2006)
Journal Article
Nasiri, H. R.; Madej, G. M.; Lancaster, C. R. D.; Schwalbe, H.; Bolte, M.: 2-Hydroxy-2-3-(3-oxobutyl)naphtalene-1,4-dione. Acta Crystallographica, Section C: Crystal Structure Communications C62 (12), pp. 0671 - 0673 (2006)
Journal Article
Zeder-Lutz, G.; Cherouati, N.; Reinhart, C.; Pattus, F.; Wagner, R.: Dot-blot immunodetection as a versatile and high-throughput assay to evaluate recombinant GPCRs produced in the yeast Pichia pastoris. Protein Expression and Purification 50 (1), pp. 118 - 127 (2006)
Journal Article
Peng, G.; Bostina, M.; Radermacher, M.; Rais, I.; Karas, M.; Michel, H.: Biochemical and electron microscopic characterization of the F1F0 ATP Synthase from the hyperthermophilic eubacterium Aquifex aeolicus. FEBS Letters 580 (25), pp. 5934 - 5940 (2006)
Journal Article
Madej, M. G.; Nasiri, H. R.; Hilgendorff, N. S.; Schwalbe, H.; Lancaster, C. R. D.: Evidence for transmembrane proton transfer in a dihaem-containing membrane protein complex. The EMBO Journal 25 (20), pp. 4963 - 4970 (2006)
Journal Article
Shukla, A. K.; Haase, W.; Reinhart, C.; Michel, H.: Functional Overexpression and Characterization of Human Bradykinin Subtype 2 Receptor in Insect Cells Using the Baculovirus System. Journal of Cellular Biochemistry 99 (3), pp. 868 - 877 (2006)
Journal Article
Shukla, A. K.; Reinhart, C.; Michel, H.: Comparative analysis of the human angiotensin II type 1a receptor heterologously produced in insect cells and mammalian cells. Biochemical and Biophysical Research Communications 349 (1), pp. 6 - 14 (2006)
Journal Article
Herzog, E.; Frigato, T.; Helms, V.; Lancaster, C. R. D.: Energy Barriers of Proton Transfer Reactions Between Amino Acid Side Chain Analogs and Water from ab initio Calculations. Journal of Computational Chemistry 27 (13), pp. 1534 - 1547 (2006)
Journal Article
Screpanti, E.; Padan, E.; Rimon, A.; Michel, H.; Hunte, C.: Crucial Steps in the Structure Determination of the Na+/H+ Antiporter NhaA in its Native Conformation. Journal of Molecular Biology (London) 362 (2), pp. 192 - 202 (2006)
Journal Article
Surade, S.; Klein, M.; Stolt-Bergner, P. C.; Muenke, C.; Roy, A.; Michel, H.: Comparative analysis and "expression space" coverage of the production of prokaryotic membrane proteins for structural genomics. Protein Science 15 (9), pp. 2178 - 2189 (2006)
Journal Article
Lancaster, C. R. D.; Haas, A.; Madej, M. G.; Mileni, M.: Recent progress on obtaining theoretical and experimental support for the "E-pathway hypothesis" of coupled transmembrane electron and proton transfer in dihaem-containing quinol:fumarate reductase. Biochimica et Biophysica Acta, Bioenergetics 1757 (8), pp. 988 - 995 (2006)
Journal Article
Wenz, T.; Hellwig, P.; MaxMillan, F.; Meunier, B.; Hunte, C.: Probing the Role of E272 in Quinol Oxidation of Mitochondrial Complex III. Biochemistry 45 (30), pp. 9042 - 9052 (2006)
Journal Article
Shukla, A. K.; Reinhart, C.; Michel, H.: Dimethylsulphoxide as a tool to increase functional expression of heterologously produced GPCRs in mammalian cells. FEBS Letters 580 (17), pp. 4261 - 4265 (2006)
Journal Article
Lundstrom, K.; Wagner, R.; Reinhart, C.; Desmyter, A.; Cherouati, N.; Magnin, T.; Zeder-Lutz, G.; Courtot, M.; Prual, C.; André, N. et al.; Hassaine, G.; Michel, H.; Cambillau, C.; Pattus, F.: Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems. Journal of Structural and Functional Genomics 7 (2), pp. 77 - 91 (2006)
Journal Article
Pilak, O.; Mamat, B.; Vogt, S.; Hagemeier, C. H.; Thauer, R. K.; Shima, S.; Vonrhein, C.; Warkentin, E.; Ermler, U.: The Crystal Structure of the Apoenzyme of the Iron-Sulphur Cluster-free Hydrogenase. Journal of Molecular Biology (London) 358 (3), pp. 798 - 809 (2006)
Journal Article
André, N.; Cherouati, N.; Prual, C.; Steffan, T.; Zeder-Lutz, G.; Magnin, T.; Pattus, F.; Michel, H.; Wagner, R.; Reinhart, C.: Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Science 15 (5), pp. 1115 - 1126 (2006)
Journal Article
Shukla, A. K.; Haase, W.; Reinhart, C.; Michel, H.: Biochemical and pharmacological characterization of the human bradykinin subtype 2 receptor produced in mammalian cells using the Semliki Forest virus system. Biological Chemistry 387 (5), pp. 569 - 576 (2006)
Journal Article
Ma, X.; Panjikar, S.; Koepke, J.; Loris, E.; Stöckigt, J.: The Structure of Rauvolfia serpentina Strictosidine Synthase Is a Novel Six-Bladed β-Propeller Fold in Plant Proteins. The Plant Cell 18 (4), pp. 907 - 920 (2006)
Journal Article
Mileni, M.; MacMillan, F.; Tziatzios, C.; Zwickers, K.; Haas, A.; Mäntele, W.; Simoni, J.; Lancaster, C. R. D.: Heterologous production in Wolinella succinogenes and characterization of the quinol:fumarate reductase enzymes from Helicobacter pylori and Campylobacter jejuni. Biochemical Journal 395 (1), pp. 191 - 201 (2006)
Journal Article
Acharya, P.; Warkentin, E.; Ermler, U.; Thauer, R. K.; Shima, S.: The structure of a Formylmethanofuran: Tetrahydromethanopterin Formyltransferase in Complex with its Coenzymes. Journal of Molecular Biology (London) 357 (3), pp. 870 - 879 (2006)
Journal Article
Schiffer, A.; Fritz, G.; Kroneck, P. M.H.; Ermler, U.: Reaction Mechanism of the Iron-Sulfur Flavoenzyme Adenosine-5 '-Phosphosulfate Reductase Based on the Structural Characterization of Different Enzymatic States. Biochemistry 45 (9), pp. 2960 - 2967 (2006)
Journal Article
Olkhova, E.; Hunte, C.; Screpanti, E.; Padan, E.; Michel, H.: Multiconformation continuum electrostatics analysis of the NhaA Na+/H+ antiporter of Escherichia coli with functional implications. Proceedings of the National Academy of Sciences of the United States of America 103 (8), pp. 2629 - 2634 (2006)
Journal Article
MacMillan, F.; Budiman, K.; Angerer, H.; Michel, H.: The role of tryptophan 272 in the Paracoccus denitrificans cytochrome c oxidase. FEBS Letters 580 (5), pp. 1345 - 1349 (2006)
Journal Article
Hassaine, G.; Wagner, R.; Kempf, J.; Cherouati, N.; Hassaine, N.; Prual, C.; André, N.; Reinhart, C.; Pattus, F.; Lundstrom, K.: Semliki Forest Virus vectors for overexpression of 101 G Protein-coupled receptors in mammalian host cells. Protein Expression and Purification 45 (2), pp. 343 - 351 (2006)
Journal Article
Hielscher, R.; Wenz, T.; Stolpe, S.; Hunte, C.; Friedrich, T.; Hellwig, P.: Monitoring Redox-Dependent Contribution of Lipids in Fourier Tranform Infrared Difference Spectra of Complex I from Escherichia coli. Biopolymers 82 (4), pp. 291 - 294 (2006)

Book Chapter (2)

2006
Book Chapter
Dierl, S.; Reinhart, C.; Reiländer, H.: Das Pichia pastoris Expressionssystem. In: Gentechnische Methoden, Eine Sammlung von Arbeitsanleitungen für das molekularbiologische Labor, 4 Ed., pp. 335 - 352 (Ed. Jansohn, M.). Spektrum Akademischer Verlag, München, Germany (2006)
Book Chapter
Reinhart, C.; Krettler, C.: Expression of membrane proteins in yeasts. In: Structural Genomics on Membrane Proteins, pp. 115 - 152 (Ed. Lundstrom, K. H.). Taylor & Francis, Boca Raton, FL, USA (2006)

Thesis - PhD (6)

2006
Thesis - PhD
Prual, C.: Production of the human adenosine A2A receptor in Pichia pastoris, its solubilization and purification, and the selection of a specific single-chain Fv fragment by phage display. Dissertation, Johann Wolfgang Goethe-University, Frankfurt am Main (2006)
Thesis - PhD
André, N.: Enhancement of the Production of Functional G Protein-Coupled Receptors in the yeast Pichia pastoris and Purification of the Rat Neurokinin 2 Receptor. Dissertation, Johann Wolfgang Goethe-University, Frankfurt am Main (2006)
Thesis - PhD
Shukla, A. K.: Heterologous Production, Characterization and Isolation of Selected G Protein-Coupled Receptors for Structural Studies. Dissertation, Johann Wolfgang Goethe-University, Frankfurt am Main (2006)
Thesis - PhD
Screpanti, E.: Structure of NhaA, a Na+/H+ antiporter from Escherichia coli and insights into mechanism of action and regulation by pH. Dissertation, Johann Wolfgang Goethe-University, Frankfurt am Main (2006)
Thesis - PhD
Solmaz, S.: The Interacting of the Cytochrome bc1 Complex with its Substrate Cytochrome c: High Resolution Structure and Implications for Transient Binding. Dissertation, Johann Wolfgang Goethe-University, Frankfurt am Main (2006)
Thesis - PhD
Huo, D.: Heterologe Expression des humanen β2-adrenergen Rezeptors im Semliki Forest Virus-Expressionssystem. Dissertation, Johann Wolfgang Goethe-University, Frankfurt am Main (2006)

Thesis - Diploma (2)

2006
Thesis - Diploma
Lößner, C.: Isolierung und Charakterisierung von rekombinanten NCS -Transportern in Escherichia coli. Diploma, Johannes Gutenberg-Universität, Mainz (2006)
Thesis - Diploma
Müller, F.: Produktion der C4-Dicarboxylat-Transporter DcuA, DcuB, DcuC und "Transporter" aus Salmonella typhimurium in Escherichia coli. Diploma, Johann Wolfgang Goethe-Universität, Frankfurt am Main (2006)
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