Martin Beck: Molekulare Soziologie

Martin Beck: Molekulare Soziologie

Die Forschung in dieser Gruppe kombiniert biochemische Ansätze, Proteomik und Kryo-Elektronenmikroskopie, um große makromolekulare Anordnungen zu untersuchen.

Nuclear pores dilate and constrict in cellulo

Nuclear pores dilate and constrict in cellulo

Zimmerli, C. E., Allegretti, M., et al. (Science 2021)
 

Cone-shaped HIV-1 capsids are transported through intact nuclear pores

Cone-shaped HIV-1 capsids are transported through intact nuclear pores

Zila, Margiotta, et al. Cell (2021)

In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges

In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges

Turoňová, Sikora, Schürmann, et al. Science (2020)

In cell architecture of the nuclear pore complex and snapshots of its turnover

In cell architecture of the nuclear pore complex and snapshots of its turnover

Allegretti, Zimmerli, et al. Nature (2020)

Benchmarking tomographic acquisition schemes for high-resolution structural biology.

Benchmarking tomographic acquisition schemes for high-resolution structural biology.

Turoňová et al., Nature Communications (2020)

Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis

Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis

Hampoelz, Schwarz, et al. Cell (2019)

In situ structural analysis of the human nuclear pore complex

In situ structural analysis of the human nuclear pore complex

von Appen et al., Nature (2015)

Disentangling Genetic and Environmental Effects on the Proteotypes of Individuals

Disentangling Genetic and Environmental Effects on the Proteotypes of Individuals

Romanov et al., Cell (2019)

Pervasive Thermal Stability Variation during the Cell Cycle

Pervasive Thermal Stability Variation during the Cell Cycle

Becher, Andres-Pons, Romanov, Stein, et al. Cell (2018)

Molecular architecture of the inner ring scaffold of the human nuclear pore complex

Molecular architecture of the inner ring scaffold of the human nuclear pore complex

Kosinski, Mosalaganti, von Appen, et al. Science (2016)

In situ architecture of the algal nuclear pore complex

In situ architecture of the algal nuclear pore complex

Mosalaganti, Kosinski, Albert, Schaffer et al., Nature Communications (2018)

Pre-assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development

Pre-assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development

Hampoelz et al., Cell (2016)

Integrated structural analysis of the human nuclear pore complex scaffold

Integrated structural analysis of the human nuclear pore complex scaffold

Bui, von Appen, et al. Cell (2013)

Forschungsschwerpunkte

 

Funktionelle Zellmodule werden bereits häufig in vitro charakterisiert. Über ihr Zusammenspiel und ihre räumlich-zeitliche Anordnung im Kontext lebender Zellen, d.h. Ihre "molekulare Soziologie", ist jedoch relativ wenig bekannt. Wir verwenden integrative strukturbiologische in-situ-Techniken, um die Struktur, Funktion und Anordnung sehr großer makromolekularer Komplexe in ihrer natürlichen Umgebung zu untersuchen. Wir stützen uns auf ein vielfältiges methodisches Repertoire, einschließlich Kryo-EM, Strukturproteomik, Biochemie, Bildgebung, Tiermodellsysteme und Computermodellierung (Robinson et al., Nature 2007; Beck und Baumeister, TiCB 2016 ).


Projekte


Ausgewählte Publikationen (Google Scholar Link)

1.
Zimmerli C. E., Allegretti M., Rantos V., Goetz S. K., Obarska-Kosinska A., Zagoriy I., Halavatyi A., Hummer G., Mahamid J., Kosinski J., Beck M.
 
Nuclear pores dilate and constrict in cellulo.
Science. 2021 Nov 11; 374 (6573). doi: 10.1126/science.abd9776
 
2.
Zila, V., Margiotta, E., Turoňová, B., Müller, T.G., Zimmerli, C.E., Mattei, S., Allegretti, M., Börner, K., Rada, J., Müller, B., Lusic, M., Kräusslich, H.G., Beck, M.
Cone-shaped HIV-1 capsids are transported through intact nuclear pores.
Cell. 2021 Feb 10; 184, 1-15. doi:10.1016/j.cell.2021.01.025.
3.
Allegretti M., Zimmerli C.E., Rantos V., Wilfling F., Ronchi P., Fung H.K.H., Lee CW., Hagen W., Turonova B., Karius K., Börmel M., Zhang X., Müller C.W., Schwab Y., Mahamid J., Pfander B., Kosinski J., Beck M. 
In-cell architecture of the nuclear pore complex and snapshots of its turnover. 
Nature. 2020 Sept 02; 586,796-800. doi: 10.1038/s41586-020-2670-5.
4.
Turoňová B, Sikora M, Schürmann C, Hagen WJH, Welsch S, Blanc FEC, von Bülow S, Gecht M, Bagola K, Hörner C, v Zandbergen G, Landry J, Doimo de Azevedo NT, Mosalaganti S, Schwarz A, Covino R, Mühlebach MD, Hummer G, Krijnse Locker J, Beck M.
In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges.
Science. 2020 Oct 9; 370, 203-208. doi: 10.1126/science.abd5223.
5.
Hampoelz B., Schwarz A., Ronchi P., Bragulat-Teixidor H., Tischer C., Gaspar I., Ephrussi A., Schwab Y., Beck M.
Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis.
Cell. 2019 Oct 17;179(3):671-686.e17. doi: 10.1016/j.cell.2019.09.022.
6.
 
Romanov N., Kuhn M., Aebersold R., Ori A., Beck M., Bork P.
Disentangling Genetic and Environmental Effects on the Proteotypes of Individuals.
Cell. 2019 May 16;177(5):1308-1318.e10. doi: 10.1016/j.cell.2019.03.015.
7.
Becher I., Andres-Pons A., Romanov N., Stein F., Schramm M., Baudin F., Helm D., Kurzawa N., Mateus A., Mackmull M.T., Typas A., Mueller C.W., Bork P., Beck M., Savitski M.M.
Pervasive Protein Thermal Stability Variation during the Cell Cycle.
Cell. 2018 May 31;173(6):1495-1507.e18. doi: 10.1016/j.cell.2018.03.053.
8.
Mosalaganti S., Kosinski J., Albert S., Schaffer M., Strenkert D., Salome P.A., Merchant S.S., Plitzko J.M., Baumeister W., Engel B.D., Beck M.
In situ architecture of the algal nuclear pore complex.
Nat Commun. 2018 Jun 18;9(1):2361. doi: 10.1038/s41467-018-04739-y.
9.
Mackmull M.T., Klaus B., Heinze I., Chokkalingam M., Beyer A., Russell R.B., Ori A., Beck M.
Landscape of nuclear transport receptor cargo specificity.
Mol Syst Biol. 2017 Dec 18;13(12):962. doi: 10.15252/msb.20177608.
10.
Hampoelz B., Mackmull M.T., Machado P., Ronchi P., Bui K.H., Schieber N., Santarella-Mellwig R., Necakov A., Andres-Pons A., Philippe J.M., Lecuit T., Schwab Y., Beck M.
Pre-assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development.
Cell. 2016 Jul 28;166(3):664-678. doi: 10.1016/j.cell.2016.06.015.
11.
Ori A., Iskar M., Buczak K., Kastritis P., Parca L., Andres-Pons A., Singer S., Bork P., Beck M.
Spatiotemporal variation of mammalian protein complex stoichiometries.
Genome Biol. 2016 Mar 14;17:47. doi: 10.1186/s13059-016-0912-5.
12.
Kosinski J., Mosalaganti S., von Appen A., Teimer R., DiGuilio A.L., Wan W., Bui K.H., Hagen W.J,, Briggs J.A., Glavy J.S., Hurt E., Beck M.
Molecular architecture of the inner ring scaffold of the human nuclear pore complex.
Science. 2016 Apr 15;352(6283):363-5. doi: 10.1126/science.aaf0643.
13.
von Appen A., Kosinski J., Sparks L., Ori A., DiGuilio A.L., Vollmer B., Mackmull M.T., Banterle N., Parca L., Kastritis P., Buczak K., Mosalaganti S., Hagen W., Andres-Pons A., Lemke E.A., Bork P., Antonin W., Glavy J.S., Bui K.H., Beck M.
In situ structural analysis of the human nuclear pore complex.
Nature. 2015 Oct 1;526(7571):140-143. doi: 10.1038/nature15381.
14.
Ori A., Toyama B.H., Harris M.S., Bock T., Iskar M., Bork P., Ingolia N.T., Hetzer M.W., Beck M.
Integrated Transcriptome and Proteome Analyses Reveal Organ-Specific Proteome Deterioritation in Rats.
Cell Syst 2015, 1(3), 224–237. doi: 10.1016/j.cels.2015.08.012
15.
Bui K.H., von Appen A., DiGuilio A.L., Ori A., Sparks L., Mackmull M.T., Bock T., Hagen W., Andres-Pons A., Glavy J.S., Beck M.
Integrated structural analysis of the human nuclear pore complex scaffold.
Cell. 2013 Dec 5;155(6):1233-43. doi: 10.1016/j.cell.2013.10.055.
16.
Ori A., Banterle N., Iskar M., Andres-Pons A., Escher C., Khanh Bui H., Sparks L., Solis-Mezarino V., Rinner O., Bork P., Lemke E.A., Beck M.
Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines.
Mol Syst Biol. 2013;9:648. doi: 10.1038/msb.2013.4.
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