Publikationen Ulrich Ermler
Zeitschriftenartikel (147)
2025
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Kinetic mechanisms of electron bifurcation with electron transfer flavoprotein, NADH, butyryl-CoA dehydrogenase, and ferredoxin reveal a semiquinone cycle. Journal of Biological Chemistry 301 (10), 110727 (2025)
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The RNF/NQR redox pumps: a versatile system for energy transduction in bacteria and archaea. Applied Microbiology and Biotechnology 109, 148 (2025)
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Enzymatic Birch reduction via hydrogen atom transfer at [4Fe-4S]-OH2 and [8Fe-9S] clusters. Nature Communications 16, 3236 (2025)
2024
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The phosphatase RosC from Streptomyces davaonensis is used for roseoflavin biosynthesis and has evolved to largely prevent dephosphorylation of the important cofactor riboflavin-5'-phosphate. Journal of Molecular Biology 436 (20), 168734 (2024)
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Mutational and structural studies of (βα)8-barrel fold methylene-tetrahydropterin reductases utilizing a common catalytic mechanism. Protein Science 33 (6), e5018 (2024)
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Structural and mechanistic basis of the central energy-converting methyltransferase complex of methanogenesis. PNAS 121 (14), e2315568121 (2024)
2023
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Ethoxylate Polymer-Based 96-Well Screen for Protein Crystallization. Crystals 13, 1519 (2023)
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Crystal structure of FAD-independent methylene-tetrahydrofolate reductase from Mycobacterium hassiacum. Proteins: Structure, Function, and Bioinformatics 91 (9), S. 1329 - 1340 (2023)
2022
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Purification and structural characterization of the Na+-translocating ferredoxin: NAD+ reductase (Rnf) complex of Clostridium tetanomorphum. Nature Communications 13, 6315 (2022)
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Cryo-EM structures of pentameric autoinducer-2 exporter from Escherichia coli reveal its transport mechanism. The EMBO Journal 41 (18), e109990 (2022)
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Structure-based electron-confurcation mechanism of the Ldh-EtfAB complex. eLife 11, e77095 (2022)
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The molybdenum storage protein forms and deposits distinct polynuclear tungsten oxygen aggregates. Journal of Inorganic Biochemistry 234, 111904 (2022)
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Finis tolueni: a new type of thiolase with an integrated Zn-finger subunit catalyses the final step of anaerobic toluene metabolism. The FEBS Journal 5, 166 (2022)
2021
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Rational engineering of Luminiphilus syltensis ( R)-selective amine transaminase for the acceptance of bulky substrates. Chemical Communications 57 (96), S. 12948 - 12951 (2021)
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Structural basis of cyclic 1,3-diene forming acyl-coenzyme A dehydrogenases. Chembiochem 22 (22), S. 3172 - 3177 (2021)
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The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily. Proceedings of the National Academy of Sciences of the United States of America 118 (46), e2107335118 (2021)
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Flavins in the electron bifurcation process. Archives of Biochemistry and Biophysics 701, 108796 (2021)
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Functional diversity of prokaryotic HdrA(BC) modules: Role in flavin-based electron bifurcation processes and beyond. Biochimica et Biophysica Acta, Bioenergetics 1862 (4), 148379 (2021)
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Structural and spectroscopic characterization of a HdrA‐like subunit from Hyphomicrobium denitrificans. The FEBS Journal 288 (5), S. 1664 - 1678 (2021)
2020
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Structural Basis of Hydrogenotrophic Methanogenesis. Annual Review of Microbiology 74, S. 713 - 733 (2020)
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Molecular and Low-Resolution Structural Characterization of the Na+-Translocating Glutaconyl-CoA Decarboxylase From Clostridium symbiosum. Frontiers in Microbiology 11, 480 (2020)
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The Hydride Transfer Process in NADP-dependent Methylene-tetrahydromethanopterin Dehydrogenase. Journal of Molecular Biology (London) 432 (7), S. 2042 - 2054 (2020)
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Methanogenesis involves direct hydride transfer from H2 to an organic substrate. Nature Reviews Chemistry 4 (2), S. 213 - 221 (2020)
2019
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Molybdate pumping into the molybdenum storage protein via an ATP-powered piercing mechanism. Proceedings of the National Academy of Sciences of the United States of America 52 (116), S. 26497 - 26504 (2019)
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The atomic-resolution crystal structure of activated [Fe]-hydrogenase. Nature Catalysis 2 (6), S. 537 - 543 (2019)
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Low potential enzymatic hydride transfer via highly cooperative and inversely functionalized flavin cofactorsLow potential enzymatic hydride transfer via highly cooperative and inversely functionalized flavin cofactors. Nature Communications 10, 2074 (2019)
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The Bacterial [Fe]‐Hydrogenase Paralog HmdII Uses Tetrahydrofolate Derivatives as Substrates. Angewandte Chemie International Edition in English 58 (11), S. 3506 - 3510 (2019)
2018
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The Molybdenum Storage Protein: A soluble ATP hydrolysis‐dependent molybdate pump. The FEBS Journal 285 (24), S. 4602 - 4616 (2018)
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The molybdenum storage protein - A bionanolab for creating experimentally alterable polyoxomolybdate clusters. Journal of Inorganic Biochemistry 189 (12), S. 172 - 179 (2018)
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How [Fe]-Hydrogenase from Methanothermobacter is Protected Against Light and Oxidative Stress. Angewandte Chemie International Edition in English 57 (46), S. 15056 - 15059 (2018)
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Dioxygen Sensitivity of [Fe]‐Hydrogenase in the Presence of Reducing Substrates. Angewandte Chemie International Edition in English 57 (18), S. 4917 - 4920 (2018)
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Molecular basis of the flavin‐based electron‐bifurcating caffeyl‐CoA reductase reaction. FEBS Letters 592 (3), S. 332 - 342 (2018)
2017
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The semiquinone swing in the bifurcating electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile. Nature Communications 8, 1577 (2017)
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A rare polyglycine type II-like helix motif in naturally occurring proteins. Proteins: Structure, Function, and Bioinformatics 85 (11), S. 2017 - 2023 (2017)
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Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons. Nature Communications 8, 542 (2017)
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A Water-Bridged H-Bonding Network Contributes to the Catalysis of the SAM-Dependent C-Methyltransferase HcgC. Angewandte Chemie, International Edition in English 56 (36), S. 10806 - 10809 (2017)
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Methanogenic heterodisulfide reductase (HdrABC-MvhAGD) uses two noncubane [4Fe-4S] clusters for reduction. Science 357 (6352), S. 699 - 703 (2017)
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Towards artificial methanogenesis: biosynthesis of the [Fe]-hydrogenase cofactor and characterization of the semi-synthetic hydrogenase. Faraday Discussions 198 (37), S. 37 - 58 (2017)
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The Crystal Structure of RosB: Insights into the Reaction Mechanism of the First Member of a Family of Flavodoxin-like Enzymes. Angewandte Chemie, International Edition in English 56 (4), S. 1146 - 1151 (2017)
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Structure of the acetophenone carboxylase core complex: prototype of a new class of ATP-dependent carboxylases/hydrolases. Scientific Reports 7, 39674 (2017)
2016
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Ligand binding and conformational dynamics in a flavin-based electron-bifurcating enzyme complex revealed by Hydrogen-Deuterium Exchange Mass Spectrometry. FEBS Letters 590 (24), S. 4472 - 4479 (2016)
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The methanogenic CO2 reducing-and-fixing enzyme is bifunctional and contains 46 [4Fe-4S] clusters. Science Magazine 354 (6308), S. 114 - 117 (2016)
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Molecular characterization of methanogenic N5-methyltetrahydromethanopterin: Coenzyme M methyltransferase. Biochimica et Biophysica Acta-Biomembranes 1858 (9), S. 2140 - 2144 (2016)
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Didehydroaspartate Modification in Methyl-Coenzyme M Reductase Catalyzing Methane Formation. Angewandte Chemie International Edition in English 55 (36), S. 10630 - 10633 (2016)
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Identification of HcgC as a SAM-Dependent Pyridinol Methyltransferase in [Fe]-Hydrogenase Cofactor Biosynthesis. Angewandte Chemie International Edition in English 55 (33), S. 9648 - 9651 (2016)
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MtrA of the sodium ion pumping methyltransferase binds cobalamin in a unique mode. Scientific Reports 6, 28226 (2016)
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X-ray structure of linalool dehydratase/isomerase from Castellaniella defragrans reveals enzymatic alkene synthesis. FEBS Letters 590 (9), S. 1375 - 1383 (2016)
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Structure and Function of the Unusual Tungsten Enzymes Acetylene Hydratase and Class II Benzoyl-Coenzyme A Reductase. Journal of Molecular Microbiology and Biotechnology 26 (1-3), S. 119 - 137 (2016)
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Fermentative Cyclohexane Carboxylate Formation in Syntrophus aciditrophicus. Journal of Molecular Microbiology and Biotechnology 26 (1-3), S. 165 - 179 (2016)
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Anaerobic Microbial Degradation of Hydrocarbons: From Enzymatic Reactions to the Environment. Journal of Molecular Microbiology and Biotechnology 26 (1-3), S. 5 - 28 (2016)
2015
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Insights into Flavin-based Electron Bifurcation via the NADH-dependent Reduced Ferredoxin:NADP Oxidoreductase Structure. The Journal of Biological Chemistry 290 (36), S. 21985 - 21885 (2015)
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Towards a functional identification of catalytically inactive [Fe]-hydrogenase paralogs. FEBS Journal 282 (17), S. 3412 - 3423 (2015)
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Structural basis of enzymatic benzene ring reduction. Nature Chemical Biology 11 (8), S. 586 - 591 (2015)
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Protein-pyridinol thioester precursor for biosynthesis of the organometallic acyl-iron ligand in [Fe]-hydrogenase cofactor. Nature Communications 6, e6895 (2015)
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Structure of the GcpE-HMBPP complex from Thermus thermophilius. Biochemical and Biophysical Research Communications 458 (2), S. 246 - 250 (2015)
2014
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Structural diversity of polyoxomolybdate clusters along the three-fold axis of the molybdenum storage protein. Journal of Inorganic Biochemistry 138, S. 122 - 128 (2014)
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A possible iron delivery function of the dinuclear iron center of HcgD in [Fe]-hydrogenase cofactor biosynthesis. FEBS Letters 588 (17), S. 2789 - 2793 (2014)
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The F420-Reducing [NiFe]-Hydrogenase Complex from Methanothermobacter marburgensis, the First X-ray Structure of a Group 3 Family Member. Journal of Molecular Biology (London) 426 (15), S. 2813 - 2826 (2014)
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A decade of crystallization drops: Crystallization of the cbb3 cytochrome c oxidase from Pseudomonas stutzeri. Protein Science 23 (4), S. 411 - 422 (2014)
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Studies on the Mechanism of Electron Bifurcation Catalyzed by Electron Transferring Flavoprotein (Etf) and Butyryl-CoA Dehydrogenase (Bcd) of Acidaminococcus fermentans. The Journal of Biological Chemistry 289 (8), S. 5145 - 5157 (2014)
2013
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Structure of the (E)-4-hydroxy-3-methyl-but-2-enyl-diphosphate reductase from Plasmodium falciparum. FEBS Letters 587 (24), S. 3968 - 3972 (2013)
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Advanced electron paramagnetic resonance on the catalytic iron–sulfur cluster bound to the CCG domain of heterodisulfide reductase and succinate: quinone reductase. Journal of Biological Inorganic Chemistry 18 (8), S. 905 - 915 (2013)
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Identification of the HcgB Enzyme in [Fe]-Hydrogenase-Cofactor Biosynthesis. Angewandte Chemie, International Edition in English 52 (48), S. 12555 - 12558 (2013)
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Structural, Biochemical and Genetic Characterization of Dissimilatory ATP Sulfurylase from Allochromatium vinosum. PLoS One 8 (9), S. e74707 - e74707 (2013)
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Crystal Structures of [Fe]-Hydrogenase in Complex with Inhibitory Isocyanides: Implications for the H2-Activation Site. Angewandte Chemie, International Edition in English 52 (37), S. 9656 - 9659 (2013)
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Conserving energy with sulfate around 100 °C - structure and mechanism of key metal enzymes in hyperthermophilic Archaeoglobus fulgidus. Metallomics 5 (4), S. 302 - 317 (2013)
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Structural Basis for a Bispecific NADP+ and CoA Binding Site in an Archaeal Malonyl-Coenzyme A Reductase. The Journal of Biological Chemistry 288 (9), S. 6363 - 6370 (2013)
2012
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Active site analysis of yeast flavohemoglobin based on its structure with a small ligand or econazole. The FEBS Journal 279 (24), S. 4565 - 4575 (2012)
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Structure and Catalytic Mechanism of N5,N10-Methenyltetrahydromethanopterin Cyclohydrolase. Biochemistry 51 (42), S. 8435 - 8443 (2012)
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Structure of the GcpE (IspG)-MEcPP complex from Thermus thermophilus. FEBS Letters 586 (19), S. 3452 - 3457 (2012)
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Crystal structure and biochemistry of methyl-coenzyme M reductase from Black Sea mats mediating anaerobic oxidation of methane. Biseibutsu Seitai 27 (2), S. 55 - 62 (2012)
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Nature’s Polyoxometalate Chemistry: X-ray Structure of the Mo Storage Protein Loaded with Discrete Polynuclear Mo−O Clusters. The Journal of Physical Chemistry A 134 (23), S. 9768 - 9774 (2012)
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Crystal structure of a ring-cleaving cyclohexane-1,2-dione hydrolase, a novel member of the thiamine diphosphate enzyme family. The FEBS Journal 279 (7), S. 1209 - 1219 (2012)
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Structure of a methyl-coenzyme M reductase from Black Sea mats that oxidize methane anaerobically. Nature 481 (7379), S. 98 - 101 (2012)
2011
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Cyclohexane-1,2-Dione Hydrolase from Denitrifying Azoarcus sp. Strain 22Lin, a Novel Member of the Thiamine Diphosphate Enzyme Family. Journal of Bacteriology 193 (23), S. 6760 - 6769 (2011)
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Structure and Mechanism of the Diiron Benzoyl-Coenzyme A Epoxidase BoxB. The Journal of Biological Chemistry 286 (33), S. 29241 - 29248 (2011)
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Structure and Function of [Fe]-Hydrogenase and its Iron–Guanylylpyridinol (FeGP) Cofactor. European Journal of Inorganic Chemistry 2011 (7), S. 963 - 972 (2011)
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Ribosome recycling depends on a mechanistic link between the FeS cluster domain and a conformational switch of the twin-ATPase ABCE1. Proceedings of the National Academy of Sciences of the United States of America 108 (8), S. 3228 - 3233 (2011)
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Structure of Ralstonia eutropha Flavohemoglobin in Complex with Three Antibiotic Azole Compounds. Biochemistry 50 (7), S. 1255 - 1264 (2011)
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Structure of the E-1-hydroxy-2-methyl-but-2-enyl-4-diphosphate synthase (GcpE) from Thermus thermophilus. FEBS Letters 585 (3), S. 447 - 451 (2011)
2010
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Reaction Cycle of the Dissimilatory Sulfite Reductase from Archaeoglobus fulgidus. Biochemistry 49 (41), S. 8912 - 8921 (2010)
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The Structure of cbb3 Cytochrome Oxidase Provides Insights into Proton Pumping. Science 329 (5989), S. 327 - 330 (2010)
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Structure at 1.5 Å resolution of cytochrome c552 with its flexible linker segment, a membrane-anchored protein from Paracoccus denitrificans. Acta Crystallographica. Section D: Biological Crystallography (Copenhagen) D66 (7), S. 850 - 854 (2010)
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Structural Basis for Promoting and Preventing Decarboxylation in Glutaryl-Coenzyme A Dehydrogenases. Biochemistry 49 (25), S. 5350 - 5357 (2010)
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A new structure-based classification of sulfide:quinone oxidoreductases. Proteins: Structure, Function, and Bioinformatics 78 (5), S. 1073 - 1083 (2010)
2009
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Cyclohexane-1,2-dione hydrolase: A new tool to degrade alicyclic compounds. Journal of Molecular Catalysis B: Enzymatic 61 (1-2), S. 47 - 49 (2009)
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Structural Basis of the Hydride Transfer Mechanism in F420-Dependent Methylenetetrahydromethanopterin Dehydrogenase. Biochemistry 49 (42), S. 10098 - 10105 (2009)
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The Crystal Structure of an [Fe]-Hydrogenase-Substrate Complex Reveals the Framework for H2 Activation. Angewandte Chemie, International Edition in English 48 (35), S. 6457 - 6460 (2009)
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The structure of Aquifex aeolicus sulfide:quinone oxidoreductase, a basis to understand sulfide detoxification and respiration. Proceedings of the National Academy of Sciences of the United States of America 106 (24), S. 9625 - 9630 (2009)
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The crystal structure of C176A mutated [Fe]-hydrogenase suggests an acyl-iron ligation in the active site iron complex. FEBS Letters 583 (3), S. 585 - 590 (2009)
2008
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The Crystal Structure of [Fe]-Hydrogenase Reveals the Geometry of the Active Site. Science 321 (5888), S. 572 - 575 (2008)
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Structure of the Dissimilatory Sulfite Reductase from the Hyperthermophilic Archaeon Archaeoglobus fulgidus. Journal of Molecular Biology (London) 379 (5), S. 1063 - 1074 (2008)
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Inhibitors of the Molybdenum Cofactor Containing 4-Hydroxybenzoyl-CoA Reductase. Biochemistry 47 (17), S. 4964 - 4972 (2008)
2007
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Towards Biological Supramolecular Chemistry: A Variety of Pocket-Templated Metal Oxide Cluster Nucleations in the Cavity of a Mo/W-Storage Protein. Angewandte Chemie, International Edition in English 46 (14), S. 2408 - 2413 (2007)
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Structure of coenzyme F420H2 oxidase (FprA), a di-iron flavoprotein from methanogenic Archaea catalysing the reduction of O2 to H2O. The FEBS Journal 274 (6), S. 1588 - 1599 (2007)
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Preliminary electrochemical studies of the flavohaemoprotein from Ralstonia eutropha entrapped in a film of methyl cellulose: activation of the reduction of dioxygen. Bioelectrochemistry 70 (1), S. 185 - 191 (2007)
2006
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Insight into the mechanism of biological methanol activation based on the crystal structure of the methanol-cobalamin methyltransferase complex. Proceedings of the National Academy of Sciences of the United States of America 103 (50), S. 18917 - 18922 (2006)
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The Crystal Structure of the Apoenzyme of the Iron-Sulphur Cluster-free Hydrogenase. Journal of Molecular Biology (London) 358 (3), S. 798 - 809 (2006)
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The structure of a Formylmethanofuran: Tetrahydromethanopterin Formyltransferase in Complex with its Coenzymes. Journal of Molecular Biology (London) 357 (3), S. 870 - 879 (2006)