Publikationen Hartmut Michel
Zeitschriftenartikel (258)
2023
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Detection of Known and Novel Small Proteins in Pseudomonas stutzeri Using a Combination of Bottom-Up and Digest-Free Proteomics and Proteogenomics. Analytical Chemistry 95, S. 11892 - 11900 (2023)
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Purification and characterization of eukaryotic ATP-dependent transporters homologously expressed in Pichia pastoris for structural studies by cryo-electron microscopy. Protein Expression and Purification 204, 106230 (2023)
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Dieter Oesterhelt (1940-2022): Life with light and color, pioneer of membrane protein research. Biophysics and Physicobiology 20 (Supplemental), e201010 (2023)
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Dieter Oesterhelt (1940–2022). Science 379 (6630), S. 337 (2023)
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Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum. Proceedings of the National Academy of Sciences of the United States of America 120 (5), e2216734120 (2023)
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The cryoEM structure of cytochrome bd from C. glutamicum provides novel insights into structural properties of actinobacterial terminal oxidases. Frontiers in Chemistry (2023)
2022
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Structural and functional investigation of ABC transporter STE6-2p from Pichia pastoris reveals unexpected interaction with sterol molecules. Proceedings of the National Academy of Sciences of the United States of America 126 (43), S. 8646 - 8654 (2022)
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Cryo-EM structures of pentameric autoinducer-2 exporter from Escherichia coli reveal its transport mechanism. The EMBO Journal 41 (18), e109990 (2022)
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Top-Down Identification and Sequence Analysis of Small Membrane Proteins Using MALDI-MS/MS. Journal of the American Society for Mass Spectrometry 33 (7), S. 1293 - 1302 (2022)
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Assembly and Functional Role of PACE Transporter PA2880 from Pseudomonas aeruginosa. Microbiology Spectrum 10 (2), e01453-21 (2022)
2021
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Mechanistic and structural diversity between cytochrome bd isoforms of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 118 (50), e2114013118 (2021)
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Cryo-EM structures of intermediates suggest an alternative catalytic reaction cycle for cytochrome c oxidase. Nature Communications 12, 6903 (2021)
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The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily. Proceedings of the National Academy of Sciences of the United States of America 118 (46), e2107335118 (2021)
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The cryo-EM structure of the bd oxidase from M. tuberculosis reveals a unique structural framework and enables rational drug design to combat TB. Nature Communications 12 (1), 5236 (2021)
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Electrocatalytic evidence of the diversity of the oxygen reaction in the bacterial bd oxidase from different organisms. Biochimica et Biophysica Acta, Bioenergetics 1862 (8), e148436 (2021)
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The unusual homodimer of a heme‐copper terminal oxidase allows itself to utilize two electron donors. Angewandte Chemie, International Edition in English 60 (24), S. 13323 - 13330 (2021)
2020
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Structural basis for amino acid exchange by a human heteromeric amino acid transporter. Proceedings of the National Academy of Sciences of the United States of America 117 (35), 202008111, S. 21281 - 21287 (2020)
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Synthesis and Biological Screening of new Lawson Derivatives as selective substrate‐based Inhibitors of Cytochrome bo3 Ubiquinol Oxidase from Escherichia coli. ChemMedChem 15 (14), S. 1262 - 1271 (2020)
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Isolated Heme A Synthase from Aquifex aeolicus Is a Trimer. mBio 11 (3), e02615-19 (2020)
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A 3.3 Å‐Resolution Structure of Hyperthermophilic Respiratory Complex III Reveals the Mechanism of its Thermal Stability. Angewandte Chemie, International Edition in English 59 (1), S. 343 - 351 (2020)
2019
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Characterization and X-ray structure of the NADH-dependent coenzyme A disulfide reductase from Thermus thermophilus. Biochimica et Biophysica Acta, Bioenergetics 1860 (11), e148080 (2019)
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Active site rearrangement and structural divergence in prokaryotic respiratory oxidases. Science 366 (6461), S. 100 - 104 (2019)
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Inward-facing conformation of a multidrug resistance MATE family transporter. Proceedings of the National Academy of Sciences of the United States of America 116 (25), S. 12275 - 12284 (2019)
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Identification of competitive inhibitors of the human taurine transporter TauT in a human kidney cell line. Pharmacological Reports 71 (1), S. 121 - 127 (2019)
2018
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Structural properties of the peroxiredoxin AhpC2 from the hyperthermophilic eubacterium Aquifex aeolicus. Biochimica et Biophysica Acta (BBA) - General Subjects 1862 (12), S. 2797 - 2805 (2018)
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Identification and characterization two isoforms of NADH:ubiquinone oxidoreductase from the hyperthermophilic eubacterium Aquifex aeolicus. Biochimica et Biophysica Acta, Bioenergetics 1859 (5), S. 366 - 373 (2018)
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The Xenobiotic Extrusion Mechanism of the MATE Transporter NorM_PS from Pseudomonas stutzeri. Journal of Molecular Biology (London) 430 (9), S. 1311 - 1323 (2018)
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The molecular basis of subtype selectivity of human kinin G-protein-coupled receptors. Nature Chemical Biology 13 (3), S. 284 - 290 (2018)
2017
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Ligand-induced conformational dynamics of the Escherichia coli Na+/H+ antiporter NhaA revealed by hydrogen/deuterium exchange mass spectrometry. Proceedings of the National Academy of Sciences of the United States of America 114 (44), S. 11691 - 11696 (2017)
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Pseudomonas stutzeri as an alternative host for membrane proteins. Microbial Cell Factories 16, 157 (2017)
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Subunit CcoQ is involved in the assembly of the Cbb3-type cytochrome c oxidases from Pseudomonas stutzeri ZoBell but not required for their activity. Biochimica et Biophysica Acta, Bioenergetics 1858 (3), S. 231 - 238 (2017)
2016
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Identification of the High-affinity Substrate-binding Site of the Multidrug and Toxic Compound Extrusion (MATE) Family Transporter from Pseudomonas stutzeri. The Journal of Biological Chemistry 291 (30), S. 15503 - 15514 (2016)
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Structure of a bd oxidase indicates similar mechanisms for membrane-integrated oxygen reductases. Science 352 (6285), S. 583 - 586 (2016)
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Functional characterization of solute carrier (SLC) 26/sulfate permease (SulP) proteins in membrane mimetic systems. Biochimica et Biophysica Acta-Biomembranes 1858 (4), S. 698 - 705 (2016)
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Identification and characterization of the novel subunit CcoM in the cbb3-cytochrome c oxidase from Pseudomonas stutzeri ZoBell. mBio 7 (7), S. 1 - 9 (2016)
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Cell-free synthesis of isotopically labelled peptide ligands for the functional characterization of G protein-coupled receptors. FEBS Open Bio 6 (1), S. 90 - 102 (2016)
2015
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NhaA: A Unique Structural Fold of Secondary Active Transporters. Israel Journal of Chemistry 55 (11/12), S. 1233 - 1239 (2015)
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Development of a Thermofluor assay for stability determination of membrane proteins using the Na+/H+antiporter NhaA and cytochrome c oxidase. Acta Crystallographica. Section D: Biological Crystallography (Copenhagen) 71 (5), S. 1112 - 1122 (2015)
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Electrochemistry suggests proton access from the exit site to the binuclear center in Paracoccus denitrificans cytochrome c oxidase pathway variants. FEBS Letters 589 (5), S. 565 - 568 (2015)
2014
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Evidence for Distinct Electron Transfer Processes in Terminal Oxidases from Different Origin by Means of Protein Film Voltammetry. Journal of the American Chemical Society 136 (31), S. 10821 - 11196 (2014)
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A decade of crystallization drops: Crystallization of the cbb3 cytochrome c oxidase from Pseudomonas stutzeri. Protein Science 23 (4), S. 411 - 422 (2014)
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A L-Lysine Transporter of High Stereoselectivity of the Amino Acid-Polyamine-Organocation (APC) Superfamily PRODUCTION, FUNCTIONAL CHARACTERIZATION, AND STRUCTURE MODELING. The Journal of Biological Chemistry 289 (3), S. 1377 - 1387 (2014)
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Biochemical and Biophysical Characterization of the Two Isoforms of cbb3-Type Cytochrome c Oxidase from Pseudomonas stutzeri. Journal of Bacteriology (Washington, DC) 196 (2), S. 472 - 482 (2014)
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Production of fully assembled and active Aquifex aeolicus F1FO ATP synthase in Escherichia coli. Biochimica et Biophysica Acta (BBA) - General Subjects 1840 (1), S. 34 - 40 (2014)
2013
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Resonance Raman Characterization of the Ammonia-Generated Oxo Intermediate of Cytochrome c Oxidase from Paracoccus denitrificans. Biochemistry 52 (36), S. 6197 - 6202 (2013)
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Exploiting Leishmania tarentolae cell-free extracts for the synthesis of human solute carriers. Molecular Membrane Biology 30 (4), S. 288 - 302 (2013)
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Role of the N-terminal signal peptide in the membrane insertion of Aquifex aeolicus F1F0 ATP synthase c-subunit. The FEBS Journal 280 (14), S. 3425 - 3435 (2013)
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True wild type and recombinant wild type cytochrome c oxidase from Paracoccus denitrificans show a 20-fold difference in their catalase activity. Biochimica et Biophysica Acta, Bioenergetics 1827 (3), S. 319 - 327 (2013)
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Exploring the entrance of proton pathways in cytochrome c oxidase from Paracoccus denitrificans: Surface charge, buffer capacity and redox-dependent polarity changes at the internal surface. Biochimica et Biophysica Acta, Bioenergetics 1827 (3), S. 276 - 284 (2013)
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Evaluation of cell-free protein synthesis for the crystallization of membrane proteins – a case study of the glutamate transporter family from Staphylothermus marinus. The FEBS Journal 280 (4), S. 1112 - 1125 (2013)
2012
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Isolation, functional characterization and crystallization of Aq_1259, an outer membrane protein with porin features, from Aquifex aeolicus. Biochimica et Biophysica Acta-Proteins and Proteomics 1824 (12), S. 1358 - 1365 (2012)
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Heterologous production and functional and thermodynamic characterization of cation diffusion facilitator (CDF) transporters of mesophilic and hyperthermophilic origin. Biological Chemistry 393 (7), S. 617 - 629 (2012)
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The ESFRI Instruct Core Centre Frankfurt: automated high-throughput crystallization suited for membrane proteins and more. Journal of Structural and Functional Genomics, S. 63 - 69 (2012)
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The nitrite transport protein NirC from Salmonella typhimurium is a nitrite/proton antiporter. Biochimica et Biophysica Acta-Biomembranes 1818 (5), S. 1342 - 1350 (2012)
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The Nonsense of Biofuels. Angewandte Chemie, International Edition in English 51 (11), S. 2516 - 2518 (2012)
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Net Proton Uptake Is Preceded by Multiple Proton Transfer Steps upon Electron Injection into Cytochrome c Oxidase. The Journal of Biological Chemistry 287 (11), S. 8187 - 8193 (2012)
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Heme-copper terminal oxidase using both cytochrome c and ubiquinol as electron donors. Proceedings of the National Academy of Sciences of the United States of America 109 (9), S. 3275 - 3280 (2012)
2011
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Interconversions of P and F intermediates of cytochrome c oxidase from Paracoccus denitrificans. Proceedings of the National Academy of Sciences of the United States of America 108 (10), S. 3964 - 3969 (2011)
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PICKScreens, A New Database for the Comparison of Crystallization Screens for Biological Macromolecules. Crystal Growth & Design 11 (2), S. 488 - 491 (2011)
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The high-affinity QH binding site in quinol oxidase as studied by DONUT-HYSCORE spectroscopy and density functional theory. Faraday Discussions 148, S. 315 - 344 (2011)
2010
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Characterizing a monotopic membrane enzyme. Biochemical, enzymatic and crystallization studies on Aquifex aeolicus sulfide:quinone oxidoreductase. Biochimica et Biophysica Acta-Biomembranes 1798 (11), S. 2114 - 2123 (2010)
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The Structure of cbb3 Cytochrome Oxidase Provides Insights into Proton Pumping. Science 329 (5989), S. 327 - 330 (2010)
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Structure at 1.5 Å resolution of cytochrome c552 with its flexible linker segment, a membrane-anchored protein from Paracoccus denitrificans. Acta Crystallographica. Section D: Biological Crystallography (Copenhagen) D66 (7), S. 850 - 854 (2010)
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A new structure-based classification of sulfide:quinone oxidoreductases. Proteins: Structure, Function, and Bioinformatics 78 (5), S. 1073 - 1083 (2010)
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The structure of eukaryotic and prokaryotic complex I. Journal of Structural Biology 169 (1), S. 81 - 88 (2010)
2009
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Spectral Diffusion and Electron-Phonon Coupling of the B800 BChl a Molecules in LH2 Complexes from Three Different Species of Purple Bacteria. Biophysical Journal 97 (9), S. 2604 - 2612 (2009)
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Combined computational and biochemical study reveals the importance of electrostatic interactions between the ‘‘pH sensor’’ and the cation binding site of the sodium/proton antiporter NhaA of Escherichia coli. Proteins: Structure, Function, and Bioinformatics 76 (3), S. 548 - 559 (2009)
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The structure of Aquifex aeolicus sulfide:quinone oxidoreductase, a basis to understand sulfide detoxification and respiration. Proceedings of the National Academy of Sciences of the United States of America 106 (24), S. 9625 - 9630 (2009)
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High resolution crystal structure of Paracoccus denitrificans cytochrome c oxidase: New insights into the active site and the proton transfer pathways. Biochimica et Biophysica Acta, Bioenergetics 1787 (6), S. 635 - 645 (2009)
2008
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Heterologous expression of human Neuromedin U receptor 1 and its subsequent solubilization and purification. Biochimica et Biophysica Acta-Biomembranes 1778 (10), S. 2203 - 2209 (2008)
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Epitope Mapping of Conformational Monoclonal Antibodies Specific to NhaA Na+/H+ antiporter: Structural and Functional Implications. Journal of Molecular Biology (London) 379 (3), S. 471 - 481 (2008)
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The Structure of the Neuropeptide Bradykinin Bound to the Human G-Protein Coupled Receptor Bradykinin B2 as Determined by Solid-State NMR Spectroscopy. Angewandte Chemie 120 (9), S. 1692 - 1695 (2008)
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A D-Pathway Mutation Decouples the Paracoccus denitrificans Cytochrome c Oxidase by Altering the Side-Chain Orientation of a Distant Conserved Glutamate. Journal of Molecular Biology (London) 384 (4), S. 865 - 877 (2008)
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Employing Rhodobacter sphaeroides to functionally express and purify human G protein-coupled receptors. Biological Chemistry Hoppe-Seyler 389 (1), S. 69 - 78 (2008)
2007
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C-terminal truncated cannabinoid receptor 1 co-expressed with G protein trimer in Sf9 cells exist in a precoupled state and show constitutive activity. The FEBS Journal 274 (23), S. 6106 - 6115 (2007)
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Structure of the Yeast WD40 Domain Protein Cia1, a Component Acting Late in Iron-Sulfur Protein Biogenesis. Structure 15 (10), S. 1246 - 1257 (2007)
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Protein AQ_1862 from the Hyperthermophilic Bacterium Aquifex aeolicus is a Porin and Contains Two Conductance Pathways of Different Selectivity. Biophysical Journal 93 (8), S. 2667 - 2677 (2007)
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Heterologous expression and characterization of the recombinant bradykinin B2 receptor using the methylotrophic yeast Pichia pastoris. Protein Expression and Purification 55 (1), S. 1 - 8 (2007)
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Functional analysis of cell-free produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation. The FEBS Journal 274 (13), S. 3257 - 3269 (2007)
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The Influence of Protonation States on the Dynamics of the NhaA Antiporter from Escherichia coli. Biophysical Journal 92 (11), S. 3784 - 3791 (2007)
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Heterologous expression and comparative characterization of the human neuromedin U subtype II receptor using the methylotrophic yeast Pichia pastoris and mammalian cells. The International Journal of Biochemistry & Cell Biology 39 (5), S. 931 - 942 (2007)
2006
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Biochemical and electron microscopic characterization of the F1F0 ATP Synthase from the hyperthermophilic eubacterium Aquifex aeolicus. FEBS Letters 580 (25), S. 5934 - 5940 (2006)
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Functional Overexpression and Characterization of Human Bradykinin Subtype 2 Receptor in Insect Cells Using the Baculovirus System. Journal of Cellular Biochemistry 99 (3), S. 868 - 877 (2006)
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Comparative analysis of the human angiotensin II type 1a receptor heterologously produced in insect cells and mammalian cells. Biochemical and Biophysical Research Communications 349 (1), S. 6 - 14 (2006)
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Crucial Steps in the Structure Determination of the Na+/H+ Antiporter NhaA in its Native Conformation. Journal of Molecular Biology (London) 362 (2), S. 192 - 202 (2006)
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Comparative analysis and "expression space" coverage of the production of prokaryotic membrane proteins for structural genomics. Protein Science 15 (9), S. 2178 - 2189 (2006)
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Dimethylsulphoxide as a tool to increase functional expression of heterologously produced GPCRs in mammalian cells. FEBS Letters 580 (17), S. 4261 - 4265 (2006)
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Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems. Journal of Structural and Functional Genomics 7 (2), S. 77 - 91 (2006)
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Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Science 15 (5), S. 1115 - 1126 (2006)
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Biochemical and pharmacological characterization of the human bradykinin subtype 2 receptor produced in mammalian cells using the Semliki Forest virus system. Biological Chemistry 387 (5), S. 569 - 576 (2006)
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Rates and Equilibrium of CuA to Heme a Electron Transfer in Paracoccus denitrificans Cytochrome c Oxidase. Biophysical Journal 90, S. 2131 - 2137 (2006)
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Multiconformation continuum electrostatics analysis of the NhaA Na+/H+ antiporter of Escherichia coli with functional implications. Proceedings of the National Academy of Sciences of the United States of America 103 (8), S. 2629 - 2634 (2006)
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The role of tryptophan 272 in the Paracoccus denitrificans cytochrome c oxidase. FEBS Letters 580 (5), S. 1345 - 1349 (2006)
2005
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Titration Behavior of Residues at the Entrance of the D-Pathway of Cytochrome c Oxidase from Paracoccus denitrificans Investigated by Continuum Electrostatic Calculations. Biophysical Journal 89 (4), S. 2324 - 2331 (2005)
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Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH. Nature 435 (7046), S. 1197 - 1202 (2005)
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Multivariate Analysis of Single-Molecule Spectra: Surpassing Spectral Diffusion. Physical Review Letters 94 (19), 195501 (2005)
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Expression, purification, crystallization and preliminary X-ray analysis of strictosidine glucosidase, an enzyme initiating biosynthetic pathways to a unique diversity of indole alkaloid skeletons. Proteins & Proteomics, S. 89 - 92 (2005)
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Structure at 1.3 Å Resolution of Rhodothermus marinus caa3 Cytochrome c Domain. Journal of Molecular Biology (London) 345 (5), S. 1047 - 1057 (2005)
2004
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Crystallization and preliminary X-ray crystallographic analysis of strictosidine synthase from Rauvolfia: the first member of a novel enzyme family. Biochimica et Biophysica Acta-Proteins and Proteomics 1702, S. 121 - 124 (2004)