Hartmut Michel: Molecular Membrane Biology – Department Publications
Journal Article (620)
2025
Journal Article
Kinetic mechanisms of electron bifurcation with electron transfer flavoprotein, NADH, butyryl-CoA dehydrogenase, and ferredoxin reveal a semiquinone cycle. Journal of Biological Chemistry 301 (10), 110727 (2025)
Journal Article
The RNF/NQR redox pumps: a versatile system for energy transduction in bacteria and archaea. Applied Microbiology and Biotechnology 109, 148 (2025)
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Enzymatic Birch reduction via hydrogen atom transfer at [4Fe-4S]-OH2 and [8Fe-9S] clusters. Nature Communications 16, 3236 (2025)
2024
Journal Article
α-Synuclein Deletion Impairs Platelet Function: A Role for SNARE Complex Assembly. Cells 13 (24), 2089 (2024)
Journal Article
The phosphatase RosC from Streptomyces davaonensis is used for roseoflavin biosynthesis and has evolved to largely prevent dephosphorylation of the important cofactor riboflavin-5'-phosphate. Journal of Molecular Biology 436 (20), 168734 (2024)
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Molecular mechanism of choline and ethanolamine transport in humans. Nature 630, pp. 501 - 508 (2024)
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Mutational and structural studies of (βα)8-barrel fold methylene-tetrahydropterin reductases utilizing a common catalytic mechanism. Protein Science 33 (6), e5018 (2024)
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The synergy between the PscC subunits for electron transfer to the P840 special pair in Chlorobaculum tepidum. Photosynthesis Research 160 (2-3), pp. 87 - 96 (2024)
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Structural and mechanistic basis of the central energy-converting methyltransferase complex of methanogenesis. PNAS 121 (14), e2315568121 (2024)
2023
Journal Article
Ethoxylate Polymer-Based 96-Well Screen for Protein Crystallization. Crystals 13, 1519 (2023)
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Crystal structure of FAD-independent methylene-tetrahydrofolate reductase from Mycobacterium hassiacum. Proteins: Structure, Function, and Bioinformatics 91 (9), pp. 1329 - 1340 (2023)
Journal Article
Detection of Known and Novel Small Proteins in Pseudomonas stutzeri Using a Combination of Bottom-Up and Digest-Free Proteomics and Proteogenomics. Analytical Chemistry 95, pp. 11892 - 11900 (2023)
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Dissecting the conformational complexity and mechanism of a bacterial heme transporter. Nature Chemical Biology 19 (8), pp. 992 - 1003 (2023)
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Crystal search - feasibility study of a real-time deep learning process for crystallization well images. Acta Crystallographica Section A: Foundations and Advances A79 (Part 4), pp. 331 - 338 (2023)
Journal Article
Production of Polyhydroxybutyrate by Genetically Modified Pseudomonas sp. phDV1: A Comparative Study of Utilizing Wine Industry Waste as a Carbon Source. Microorganisms 11 (16), 1592 (2023)
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Purification and characterization of eukaryotic ATP-dependent transporters homologously expressed in Pichia pastoris for structural studies by cryo-electron microscopy. Protein Expression and Purification 204, 106230 (2023)
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Dieter Oesterhelt (1940-2022): Life with light and color, pioneer of membrane protein research. Biophysics and Physicobiology 20 (Supplemental), e201010 (2023)
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Dieter Oesterhelt (1940–2022). Science 379 (6630), p. 337 (2023)
Journal Article
Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum. Proceedings of the National Academy of Sciences of the United States of America 120 (5), e2216734120 (2023)
Journal Article
The cryoEM structure of cytochrome bd from C. glutamicum provides novel insights into structural properties of actinobacterial terminal oxidases. Frontiers in Chemistry (2023)
2022
Journal Article
Purification and structural characterization of the Na+-translocating ferredoxin: NAD+ reductase (Rnf) complex of Clostridium tetanomorphum. Nature Communications 13, 6315 (2022)
Journal Article
Structural and functional investigation of ABC transporter STE6-2p from Pichia pastoris reveals unexpected interaction with sterol molecules. Proceedings of the National Academy of Sciences of the United States of America 126 (43), pp. 8646 - 8654 (2022)
Journal Article
Electrochemical Antigenic Sensor for the Diagnosis of Chronic Q Fever. Current Research in Biotechnology 4, pp. 537 - 543 (2022)
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Ligand binding and conformational dynamics of the E. coli nicotinamide nucleotide transhydrogenase revealed by hydrogen/deuterium exchange mass spectrometry. Computational and Structural Biotechnology Journal 20, pp. 5430 - 5439 (2022)
Journal Article
Cryo-EM structures of pentameric autoinducer-2 exporter from Escherichia coli reveal its transport mechanism. The EMBO Journal 41 (18), e109990 (2022)
Journal Article
Top-Down Identification and Sequence Analysis of Small Membrane Proteins Using MALDI-MS/MS. Journal of the American Society for Mass Spectrometry 33 (7), pp. 1293 - 1302 (2022)
Journal Article
Structure-based electron-confurcation mechanism of the Ldh-EtfAB complex. eLife 11, e77095 (2022)
Journal Article
The molybdenum storage protein forms and deposits distinct polynuclear tungsten oxygen aggregates. Journal of Inorganic Biochemistry 234, 111904 (2022)
Journal Article
Assembly and Functional Role of PACE Transporter PA2880 from Pseudomonas aeruginosa. Microbiology Spectrum 10 (2), e01453-21 (2022)
Journal Article
Finis tolueni: a new type of thiolase with an integrated Zn-finger subunit catalyses the final step of anaerobic toluene metabolism. The FEBS Journal 5, 166 (2022)
Journal Article
An amiloride derivative is active against the F1Fo-ATP synthase and cytochrome bd oxidase of Mycobacterium tuberculosis. Communications Biology 5 (1), 166 (2022)
2021
Journal Article
Mechanistic and structural diversity between cytochrome bd isoforms of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 118 (50), e2114013118 (2021)
Journal Article
Rational engineering of Luminiphilus syltensis ( R)-selective amine transaminase for the acceptance of bulky substrates. Chemical Communications 57 (96), pp. 12948 - 12951 (2021)
Journal Article
Short-chain aurachin D derivatives are selective inhibitors of E. coli cytochrome bd-I and bd-II oxidases. Scientific Reports 11 (1), e23852 (2021)
Journal Article
Cryo-EM structures of intermediates suggest an alternative catalytic reaction cycle for cytochrome c oxidase. Nature Communications 12, 6903 (2021)
Journal Article
Structural basis of cyclic 1,3-diene forming acyl-coenzyme A dehydrogenases. Chembiochem 22 (22), pp. 3172 - 3177 (2021)
Journal Article
The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily. Proceedings of the National Academy of Sciences of the United States of America 118 (46), e2107335118 (2021)
Journal Article
High-resolution structure and dynamics of mitochondrial complex I-Insights into the proton pumping mechanism. Science Advances 7 (46), eabj3221 (2021)
Journal Article
Expression, purification and characterization of the IcmG and IcmK proteins of the Type IVB secretion system from Coxiella burnetii. Protein Expression and Purification 186, 105905 (2021)
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The cryo-EM structure of the bd oxidase from M. tuberculosis reveals a unique structural framework and enables rational drug design to combat TB. Nature Communications 12 (1), 5236 (2021)
Journal Article
Electrocatalytic evidence of the diversity of the oxygen reaction in the bacterial bd oxidase from different organisms. Biochimica et Biophysica Acta, Bioenergetics 1862 (8), e148436 (2021)
Journal Article
Polyhydroxyalkanoate (PHA) Production in Pseudomonas sp. phDV1 Strain Grown on Phenol as Carbon Sources. Microorganisms 9 (8), 1636 (2021)
Journal Article
The unusual homodimer of a heme‐copper terminal oxidase allows itself to utilize two electron donors. Angewandte Chemie, International Edition in English 60 (24), pp. 13323 - 13330 (2021)
Journal Article
Flavins in the electron bifurcation process. Archives of Biochemistry and Biophysics 701, 108796 (2021)
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Functional diversity of prokaryotic HdrA(BC) modules: Role in flavin-based electron bifurcation processes and beyond. Biochimica et Biophysica Acta, Bioenergetics 1862 (4), 148379 (2021)
Journal Article
Structural and spectroscopic characterization of a HdrA‐like subunit from Hyphomicrobium denitrificans. The FEBS Journal 288 (5), pp. 1664 - 1678 (2021)
2020
Journal Article
In the Search of Potential Serodiagnostic Proteins to Discriminate Between Acute and Chronic Q Fever in Humans. Some Promising Outcomes. Frontiers in Cellular and Infection Microbiology 10, 557027 (2020)
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Structural Basis of Hydrogenotrophic Methanogenesis. Annual Review of Microbiology 74, pp. 713 - 733 (2020)
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Structural basis for amino acid exchange by a human heteromeric amino acid transporter. Proceedings of the National Academy of Sciences of the United States of America 117 (35), 202008111, pp. 21281 - 21287 (2020)
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Synthesis and Biological Screening of new Lawson Derivatives as selective substrate‐based Inhibitors of Cytochrome bo3 Ubiquinol Oxidase from Escherichia coli. ChemMedChem 15 (14), pp. 1262 - 1271 (2020)
Journal Article
Isolated Heme A Synthase from Aquifex aeolicus Is a Trimer. mBio 11 (3), e02615-19 (2020)
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Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium. Nature Communications 11, 2231 (2020)
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Molecular and Low-Resolution Structural Characterization of the Na+-Translocating Glutaconyl-CoA Decarboxylase From Clostridium symbiosum. Frontiers in Microbiology 11, 480 (2020)
Journal Article
The Hydride Transfer Process in NADP-dependent Methylene-tetrahydromethanopterin Dehydrogenase. Journal of Molecular Biology (London) 432 (7), pp. 2042 - 2054 (2020)
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Methanogenesis involves direct hydride transfer from H2 to an organic substrate. Nature Reviews Chemistry 4 (2), pp. 213 - 221 (2020)
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A 3.3 Å‐Resolution Structure of Hyperthermophilic Respiratory Complex III Reveals the Mechanism of its Thermal Stability. Angewandte Chemie, International Edition in English 59 (1), pp. 343 - 351 (2020)
2019
Journal Article
Molybdate pumping into the molybdenum storage protein via an ATP-powered piercing mechanism. Proceedings of the National Academy of Sciences of the United States of America 52 (116), pp. 26497 - 26504 (2019)
Journal Article
Characterization and X-ray structure of the NADH-dependent coenzyme A disulfide reductase from Thermus thermophilus. Biochimica et Biophysica Acta, Bioenergetics 1860 (11), e148080 (2019)
Journal Article
Full-length transcriptome reconstruction reveals a large diversity of RNA and protein isoforms in rat hippocampus. Nature Communications 10, 5009 (2019)
Journal Article
Active site rearrangement and structural divergence in prokaryotic respiratory oxidases. Science 366 (6461), pp. 100 - 104 (2019)
Journal Article
Proteome Analysis of Enriched Heterocysts from Two Hydrogenase Mutants from Anabaena sp. PCC 7120. Proteomics 19 (19), 1800332 (2019)
Journal Article
Rotary substates of mitochondrial ATP synthase reveal the basis of flexible F1-Fo coupling. Science 364 (6446), eaaw9128, p. 1155 (2019)
Journal Article
Inward-facing conformation of a multidrug resistance MATE family transporter. Proceedings of the National Academy of Sciences of the United States of America 116 (25), pp. 12275 - 12284 (2019)
Journal Article
Larval Zebrafish Proteome Regulation in Response to an Environmental Challenge. Proteomics 19 (14), 1900028 (2019)
Journal Article
The atomic-resolution crystal structure of activated [Fe]-hydrogenase. Nature Catalysis 2 (6), pp. 537 - 543 (2019)
Journal Article
Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder. International Journal of Molecular Sciences 20 (11), 2682 (2019)
Journal Article
Low potential enzymatic hydride transfer via highly cooperative and inversely functionalized flavin cofactorsLow potential enzymatic hydride transfer via highly cooperative and inversely functionalized flavin cofactors. Nature Communications 10, 2074 (2019)
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The Bacterial [Fe]‐Hydrogenase Paralog HmdII Uses Tetrahydrofolate Derivatives as Substrates. Angewandte Chemie International Edition in English 58 (11), pp. 3506 - 3510 (2019)
Journal Article
Cell-type-specific metabolic labeling, detection and identification of nascent proteomes in vivo. Nature Protocols 14 (2), pp. 556 - 575 (2019)
Journal Article
The structure differences of Japanese encephalitis virus SA14 and SA14-14-2 E proteins elucidate the virulence attenuation mechanism. Protein and Cell 10 (2), pp. 149 - 153 (2019)
Journal Article
Identification of competitive inhibitors of the human taurine transporter TauT in a human kidney cell line. Pharmacological Reports 71 (1), pp. 121 - 127 (2019)
Journal Article
Complete Genome Sequence of Pseudomonas sp. Strain phDV1, an Isolate Capable of Efficient Degradation of Aromatic Hydrocarbons. Microbiology Resource Announcement 8 (2), e01171-18 (2019)
2018
Journal Article
The Molybdenum Storage Protein: A soluble ATP hydrolysis‐dependent molybdate pump. The FEBS Journal 285 (24), pp. 4602 - 4616 (2018)
Journal Article
The molybdenum storage protein - A bionanolab for creating experimentally alterable polyoxomolybdate clusters. Journal of Inorganic Biochemistry 189 (12), pp. 172 - 179 (2018)
Journal Article
Structural properties of the peroxiredoxin AhpC2 from the hyperthermophilic eubacterium Aquifex aeolicus. Biochimica et Biophysica Acta (BBA) - General Subjects 1862 (12), pp. 2797 - 2805 (2018)
Journal Article
How [Fe]-Hydrogenase from Methanothermobacter is Protected Against Light and Oxidative Stress. Angewandte Chemie International Edition in English 57 (46), pp. 15056 - 15059 (2018)
Journal Article
Elucidating the control and development of skin patterning in cuttlefish. Nature 562 (7727), pp. 361 - 366 (2018)
Journal Article
Cryo-EM structure of respiratory complex I at work. eLife 7, e39213 (2018)
Journal Article
The fine art of integral membrane protein crystallization. Methods 147, pp. 150 - 162 (2018)
Journal Article
Local and global influences on protein turnover in neurons and glia. eLife 7, e34202 (2018)
Journal Article
Identification and characterization two isoforms of NADH:ubiquinone oxidoreductase from the hyperthermophilic eubacterium Aquifex aeolicus. Biochimica et Biophysica Acta, Bioenergetics 1859 (5), pp. 366 - 373 (2018)
Journal Article
Structural basis for energy transduction by respiratory alternative complex III. Nature Communications 9, 1728 (2018)
Journal Article
The Xenobiotic Extrusion Mechanism of the MATE Transporter NorM_PS from Pseudomonas stutzeri. Journal of Molecular Biology (London) 430 (9), pp. 1311 - 1323 (2018)
Journal Article
Dioxygen Sensitivity of [Fe]‐Hydrogenase in the Presence of Reducing Substrates. Angewandte Chemie International Edition in English 57 (18), pp. 4917 - 4920 (2018)
Journal Article
Tumorigenic and Antiproliferative Properties of the TALE-Transcription Factors MEIS2D and MEIS2A in Neuroblastoma. Cancer research: an official organ of the American Association for Cancer Research 78 (8), pp. 1935 - 1947 (2018)
Journal Article
The molecular basis of subtype selectivity of human kinin G-protein-coupled receptors. Nature Chemical Biology 13 (3), pp. 284 - 290 (2018)
Journal Article
Time- and polarity-dependent proteomic changes associated with homeostatic scaling at central synapses. eLife 7, e33322 (2018)
Journal Article
Molecular basis of the flavin‐based electron‐bifurcating caffeyl‐CoA reductase reaction. FEBS Letters 592 (3), pp. 332 - 342 (2018)
2017
Journal Article
Cell-type-specific metabolic labeling of nascent proteomes in vivo. Nature Biotechnology 35 (12), pp. 1196 - 1201 (2017)
Journal Article
A standardized technique for high-pressure cooling of protein crystals. Acta Crystallographica. Section D: Biological Crystallography (Copenhagen) 73 (12), pp. 997 - 1006 (2017)
Journal Article
DM9 Domain Containing Protein Functions As a Pattern Recognition Receptor with Broad Microbial Recognition Spectrum. Frontiers in immunology 8, 1607 (2017)
Journal Article
The semiquinone swing in the bifurcating electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile. Nature Communications 8, 1577 (2017)
Journal Article
A rare polyglycine type II-like helix motif in naturally occurring proteins. Proteins: Structure, Function, and Bioinformatics 85 (11), pp. 2017 - 2023 (2017)
Journal Article
Ligand-induced conformational dynamics of the Escherichia coli Na+/H+ antiporter NhaA revealed by hydrogen/deuterium exchange mass spectrometry. Proceedings of the National Academy of Sciences of the United States of America 114 (44), pp. 11691 - 11696 (2017)
Journal Article
Pseudomonas stutzeri as an alternative host for membrane proteins. Microbial Cell Factories 16, 157 (2017)
Journal Article
Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons. Nature Communications 8, 542 (2017)
Journal Article
A Water-Bridged H-Bonding Network Contributes to the Catalysis of the SAM-Dependent C-Methyltransferase HcgC. Angewandte Chemie, International Edition in English 56 (36), pp. 10806 - 10809 (2017)
Journal Article
Methanogenic heterodisulfide reductase (HdrABC-MvhAGD) uses two noncubane [4Fe-4S] clusters for reduction. Science 357 (6352), pp. 699 - 703 (2017)
Journal Article
Cryo-EM Structure of the TOM Core Complex from Neurospora crassa. Cell 170 (4), pp. 693 - 700 (2017)