Michel, Hartmut

Michel, Hartmut

Scientific member (Director)

Curriculum Vitae

  • Studies of biochemistry at Tuebingen and Munich, 1969-1975
  • Ph D., Biochemistry, University of Wuerzburg, 1977
  • Postdoc, University of Wuerzburg, 1977-1979
  • Research associate and group leader,
    Max Planck Institute of Biochemistry, 1979-1987
  • Habilitation, University of Munich, 1986
  • Director, Max Planck Institute of Biophysics,
    department of molecular membrane biology,
    since 1987
  • Adjunct Professor, University of Frankfurt,
    since 1989
Prizes and Awards (Selection):
  • 1986 Leibniz Prize of the Deutsche Forschungsgemeinschaft
  • 1988 Otto-Bayer-Prize (with Johann Deisenhofer) 
  • 1988 Nobel Prize in Chemistry (with Johann Deisenhofer and Robert Huber) 
  • 2008  Keilin Medal of the British Biochemical Society 
  • Honorary doctorates of the Universities of Wuerzburg and Bologna 
  • Honorary and visiting professorships of the Imperial College (London), 
  • IIT Madras at Chennai, IIT Mumbai, Tongji University Shanghai, 
  • Jiaotong University Xian, Tsinghua University Beijing

Publications Hartmut Michel 

Journal Article (202)

2020
Journal Article
Wu, D.; Grund, T. N.; Welsch, S.; Mills, D.; Michel, M.; Safarian, S.; Michel, H.: Structural basis for amino acid exchange by a human heteromeric amino acid transporter. Proceedings of the National Academy of Sciences of the United States of America 117, 202008111 (2020)
Journal Article
Elamri, I.; Radloff, M.; Hohmann, K. F.; Nimbarte, V. D.; Nasiri, H. R.; Bolte, M.; Safarian, S.; Michel, H.; Schwalbe, H.: Synthesis and Biological Screening of new Lawson Derivatives as selective substrate‐based Inhibitors of Cytochrome bo3 Ubiquinol Oxidase from Escherichia coli. ChemMedChem 15 (14), pp. 1262 - 1271 (2020)
Journal Article
Zeng, H.; Zhu, G.; Zhang, S.; Li, X.; Martin, J.; Morgner, N.; Sun, F.; Peng, G.; Xie, H.; Michel, H.: Isolated Heme A Synthase from Aquifex aeolicus Is a Trimer. mBio 11 (3), e02615-19 (2020)
Journal Article
Zhu, G.; Zeng, H.; Zang, S.; Juli, J.; Pang, X.; Hoffman, J.; Zhang, Y.; Morgner, N.; Zhu, Y.; Peng, G. et al.; Michel, H.; Sun, F.: A 3.3 Å‐Resolution Structure of Hyperthermophilic Respiratory Complex III Reveals the Mechanism of its Thermal Stability. Angewandte Chemie, International Edition in English 59 (1), pp. 343 - 351 (2020)
2019
Journal Article
Lencina, A. M.; Koepke, J.; Preu, J.; Münke, C.; Gennis, R. B.; Michel, H.; Schurig-Briccio, L. A.: Characterization and X-ray structure of the NADH-dependent coenzyme A disulfide reductase from Thermus thermophilus. Biochimica et Biophysica Acta, Bioenergetics 1860 (11), e148080 (2019)
Journal Article
Safarian, S.; Hahn, A.; Mills, D.; Radloff, M.; Eisinger, M. L.; Nikolaev, A.; Meier-Credo, J.; Melin, F.; Miyoshi, H.; Gennis, R.B. et al.; Sakamoto, J.; Langer, J. D.; Hellwig, P.; Kühlbrandt, W.; Michel, H.: Active site rearrangement and structural divergence in prokaryotic respiratory oxidases. Science 366 (6461), pp. 100 - 104 (2019)
Journal Article
Zakrzewska, S.; Mehdipour, A. R.; Malviya, V. N.; Nonaka, T.; Koepke, J.; Muenke, C.; Hausner, W.; Hummer, G.; Safarian, S.; Michel, H.: Inward-facing conformation of a multidrug resistance MATE family transporter. Proceedings of the National Academy of Sciences of the United States of America 116 (25), pp. 12275 - 12284 (2019)
Journal Article
Richter, M.; Moroniak, S. J.; Michel, H.: Identification of competitive inhibitors of the human taurine transporter TauT in a human kidney cell line. Pharmacological Reports 71 (1), pp. 121 - 127 (2019)
2018
Journal Article
Liu, W.; Liu, A.; Gao, H.; Wang, Q.; Wang, L.; Warkentin, E.; Rao, Z.; Michel, H.; Peng, G.: Structural properties of the peroxiredoxin AhpC2 from the hyperthermophilic eubacterium Aquifex aeolicus. Biochimica et Biophysica Acta (BBA) - General Subjects 1862 (12), pp. 2797 - 2805 (2018)
Journal Article
Peng, G.; Meyer, B.; Sokolova, L.; Liu, W.; Bornemann, S.; Juli, J.; Zwicker, K.; Karas, M.; Brutschy, B.; Michel, H.: Identification and characterization two isoforms of NADH:ubiquinone oxidoreductase from the hyperthermophilic eubacterium Aquifex aeolicus. Biochimica et Biophysica Acta, Bioenergetics 1859 (5), pp. 366 - 373 (2018)
Journal Article
Eisinger, M. L.; Nie, L.; Dörrbaum, A. R.; Langer, J. D.; Michel, H.: The Xenobiotic Extrusion Mechanism of the MATE Transporter NorM_PS from Pseudomonas stutzeri. Journal of Molecular Biology (London) 430 (9), pp. 1311 - 1323 (2018)
Journal Article
Joedicke, L.; Mao, J.; Kuenze, G.; Reinhart, C.; Kalavacherla, T.; Jonker, H. R.A.; Richter, C.; Schwalbe, H.; Meiler, J.; Preu, J. et al.; Michel, H.; Glaubitz, C.: The molecular basis of subtype selectivity of human kinin G-protein-coupled receptors. Nature Chemical Biology 13 (3), pp. 284 - 290 (2018)
2017
Journal Article
Eisinger, M. L.; Dörrbaum, A. R.; Michel, H.; Padan, E.; Langer, J. D.: Ligand-induced conformational dynamics of the Escherichia coli Na+/H+ antiporter NhaA revealed by hydrogen/deuterium exchange mass spectrometry. Proceedings of the National Academy of Sciences of the United States of America 114 (44), pp. 11691 - 11696 (2017)
Journal Article
Sommer, M.; Xie, H.; Michel, H.: Pseudomonas stutzeri as an alternative host for membrane proteins. Microbial Cell Factories 16, 157 (2017)
Journal Article
Kohlstädt, M.; Buschmann, S.; Langer, J. D.; Xie, H.; Michel, H.: Subunit CcoQ is involved in the assembly of the Cbb3-type cytochrome c oxidases from Pseudomonas stutzeri ZoBell but not required for their activity. Biochimica et Biophysica Acta, Bioenergetics 1858 (3), pp. 231 - 238 (2017)
2016
Journal Article
Laiyin, N.; Grell, E.; Malviya, V. N.; Xie, H.; Wang, J.; Michel, H.: Identification of the High-affinity Substrate-binding Site of the Multidrug and Toxic Compound Extrusion (MATE) Family Transporter from Pseudomonas stutzeri. The Journal of Biological Chemistry 291 (30), pp. 15503 - 15514 (2016)
Journal Article
Safarian, S.; Rajendran, C.; Müller, H.; Preu, J.; Langer, J. D.; Ovchinnikov, S.; Hirose, T.; Kusumoto, T.; Sakamoto, J.; Michel, H.: Structure of a bd oxidase indicates similar mechanisms for membrane-integrated oxygen reductases. Science 352 (6285), pp. 583 - 586 (2016)
Journal Article
Srinivasan, L.; Baars, T. L.; Fendler, K.; Michel, H.: Functional characterization of solute carrier (SLC) 26/sulfate permease (SulP) proteins in membrane mimetic systems. Biochimica et Biophysica Acta-Biomembranes 1858 (4), pp. 698 - 705 (2016)
Journal Article
Kohlstädt, M.; Buschmann, S.; Xie, H.; Resemann, A.; Warkentin, E.; Langer, J. D.; Michel, H.: Identification and characterization of the novel subunit CcoM in the cbb3-cytochrome c oxidase from Pseudomonas stutzeri ZoBell. mBio 7 (7), pp. 1 - 9 (2016)
Journal Article
Joedicke, L.; Trenker, R.; Langer, J. D.; Michel, H.; Preu, J.: Cell-free synthesis of isotopically labelled peptide ligands for the functional characterization of G protein-coupled receptors. FEBS Open Bio 6 (1), pp. 90 - 102 (2016)
2015
Journal Article
Padan, E.; Michel, H.: NhaA: A Unique Structural Fold of Secondary Active Transporters. Israel Journal of Chemistry 55 (11/12), pp. 1233 - 1239 (2015)
Journal Article
Kohlstädt, M.; von der Hocht, I.; Hilbers, F.; Thielmann, Y.; Michel, H.: Development of a Thermofluor assay for stability determination of membrane proteins using the Na+/H+antiporter NhaA and cytochrome c oxidase. Acta Crystallographica. Section D: Biological Crystallography (Copenhagen) 71 (5), pp. 1112 - 1122 (2015)
Journal Article
Meyer, T.; Melin, F.; Richter, O.-M.H.; Ludwig, B.; Kannt, A.; Müller, H.; Michel, H.; Hellwig, P.: Electrochemistry suggests proton access from the exit site to the binuclear center in Paracoccus denitrificans cytochrome c oxidase pathway variants. FEBS Letters 589 (5), pp. 565 - 568 (2015)
2014
Journal Article
Meyer, T.; Melin, F.; Xie, H.; von der Hocht, I.; Choi, S. K.; Noor, M. R.; Michel, H.; Gennis, R. B.; Soulimane, T.; Hellwig, P.: Evidence for Distinct Electron Transfer Processes in Terminal Oxidases from Different Origin by Means of Protein Film Voltammetry. Journal of the American Chemical Society 136 (31), pp. 10821 - 11196 (2014)
Journal Article
Buschmann, S.; Richers, S.; Ermler, U.; Michel, H.: A decade of crystallization drops: Crystallization of the cbb3 cytochrome c oxidase from Pseudomonas stutzeri. Protein Science 23 (4), pp. 411 - 422 (2014)
Journal Article
Kaur, J.; Olkhova, E.; Malviya, V. N.; Grell, E.; Michel, H.: A L-Lysine Transporter of High Stereoselectivity of the Amino Acid-Polyamine-Organocation (APC) Superfamily PRODUCTION, FUNCTIONAL CHARACTERIZATION, AND STRUCTURE MODELING. The Journal of Biological Chemistry 289 (3), pp. 1377 - 1387 (2014)
Journal Article
Xie, H.; Buschmann, S.; Langer, J. D.; Ludwig, B.; Michel, H.: Biochemical and Biophysical Characterization of the Two Isoforms of cbb3-Type Cytochrome c Oxidase from Pseudomonas stutzeri. Journal of Bacteriology (Washington, DC) 196 (2), pp. 472 - 482 (2014)
Journal Article
Zhang, C.; Allegretti, M.; Vonck, J.; Langer, J.; Marcia, M.; Peng, G.; Michel, H.: Production of fully assembled and active Aquifex aeolicus F1FO ATP synthase in Escherichia coli. Biochimica et Biophysica Acta (BBA) - General Subjects 1840 (1), pp. 34 - 40 (2014)
2013
Journal Article
Kozuch, J.; von der Hocht, I.; Hilbers, F.; Michel, H.; Weidinger, I. M.: Resonance Raman Characterization of the Ammonia-Generated Oxo Intermediate of Cytochrome c Oxidase from Paracoccus denitrificans. Biochemistry 52 (36), pp. 6197 - 6202 (2013)
Journal Article
Ruehrer, S.; Michel, H.: Exploiting Leishmania tarentolae cell-free extracts for the synthesis of human solute carriers. Molecular Membrane Biology 30 (4), pp. 288 - 302 (2013)
Journal Article
Zhang, C.; Marcia, M.; Langer, J. D.; Peng, G.; Michel, H.: Role of the N-terminal signal peptide in the membrane insertion of Aquifex aeolicus F1F0 ATP synthase c-subunit. The FEBS Journal 280 (14), pp. 3425 - 3435 (2013)
Journal Article
Hilbers, F.; von der Hocht, I.; Ludwig, B.; Michel, H.: True wild type and recombinant wild type cytochrome c oxidase from Paracoccus denitrificans show a 20-fold difference in their catalase activity. Biochimica et Biophysica Acta, Bioenergetics 1827 (3), pp. 319 - 327 (2013)
Journal Article
Kirchberg, K.; Michel, H.; Alexiev, U.: Exploring the entrance of proton pathways in cytochrome c oxidase from Paracoccus denitrificans: Surface charge, buffer capacity and redox-dependent polarity changes at the internal surface. Biochimica et Biophysica Acta, Bioenergetics 1827 (3), pp. 276 - 284 (2013)
Journal Article
Jaehme, M.; Michel, H.: Evaluation of cell-free protein synthesis for the crystallization of membrane proteins – a case study of the glutamate transporter family from Staphylothermus marinus. The FEBS Journal 280 (4), pp. 1112 - 1125 (2013)
2012
Journal Article
Wang, T.; Langer, J. D.; Peng, G.; Michel, H.: Isolation, functional characterization and crystallization of Aq_1259, an outer membrane protein with porin features, from Aquifex aeolicus. Biochimica et Biophysica Acta-Proteins and Proteomics 1824 (12), pp. 1358 - 1365 (2012)
Journal Article
Goswami, D.; Kaur, J.; Surade, S.; Grell, E.; Michel, H.: Heterologous production and functional and thermodynamic characterization of cation diffusion facilitator (CDF) transporters of mesophilic and hyperthermophilic origin. Biological Chemistry 393 (7), pp. 617 - 629 (2012)
Journal Article
Thielmann, Y.; Koepke, J.; Michel, H.: The ESFRI Instruct Core Centre Frankfurt: automated high-throughput crystallization suited for membrane proteins and more. Journal of Structural and Functional Genomics, pp. 63 - 69 (2012)
Journal Article
Rycovska, A.; Hatahet, L.; Fendler, K.; Michel, H.: The nitrite transport protein NirC from Salmonella typhimurium is a nitrite/proton antiporter. Biochimica et Biophysica Acta-Biomembranes 1818 (5), pp. 1342 - 1350 (2012)
Journal Article
Michel, H.: The Nonsense of Biofuels. Angewandte Chemie, International Edition in English 51 (11), pp. 2516 - 2518 (2012)
Journal Article
Kirchberg, K.; Michel, H.; Alexiev, U.: Net Proton Uptake Is Preceded by Multiple Proton Transfer Steps upon Electron Injection into Cytochrome c Oxidase. The Journal of Biological Chemistry 287 (11), pp. 8187 - 8193 (2012)
Journal Article
Gao, Y.; Meyer, B.; Sokolova, L.; Zwicker, K.; Karas, M.; Brutschy, B.; Peng, G.; Michel, H.: Heme-copper terminal oxidase using both cytochrome c and ubiquinol as electron donors. Proceedings of the National Academy of Sciences of the United States of America 109 (9), pp. 3275 - 3280 (2012)
2011
Journal Article
von der Hocht, I.; van Wonderen, J. H.; Hilbers, F.; Angerer, H.; MacMillan, F.; Michel, H.: Interconversions of P and F intermediates of cytochrome c oxidase from Paracoccus denitrificans. Proceedings of the National Academy of Sciences of the United States of America 108 (10), pp. 3964 - 3969 (2011)
Journal Article
Hedderich, T.; Marcia, M.; Köpke, J.; Michel, H.: PICKScreens, A New Database for the Comparison of Crystallization Screens for Biological Macromolecules. Crystal Growth & Design 11 (2), pp. 488 - 491 (2011)
Journal Article
MacMillan, F.; Kacprzak, S.; Hellwig, P.; Grimaldi, S.; Michel, H.; Kaupp, M.: The high-affinity QH binding site in quinol oxidase as studied by DONUT-HYSCORE spectroscopy and density functional theory. Spectroscopy, Theory and Mechanism in Bioinorganic Chemistry, pp. 315 - 344 (2011)
2010
Journal Article
Marcia, M.; Langer, J. D.; Parcej, D.; Vogel, V.; Peng, G.; Michel, H.: Characterizing a monotopic membrane enzyme. Biochemical, enzymatic and crystallization studies on Aquifex aeolicus sulfide:quinone oxidoreductase. Biochimica et Biophysica Acta-Biomembranes 1798 (11), pp. 2114 - 2123 (2010)
Journal Article
Buschmann, S.; Warkentin, E.; Xie, H.; Langer, J. D.; Ermler, U.; Michel, H.: The Structure of cbb3 Cytochrome Oxidase Provides Insights into Proton Pumping. Science 329 (5989), pp. 327 - 330 (2010)
Journal Article
Rajendran, C.; Ermler, U.; Ludwig, B.; Michel, H.: Structure at 1.5 Å resolution of cytochrome c552 with its flexible linker segment, a membrane-anchored protein from Paracoccus denitrificans. Acta Crystallographica. Section D: Biological Crystallography (Copenhagen) D66 (7), pp. 850 - 854 (2010)
Journal Article
Marcia, M.; Ermler, U.; Peng, G.; Michel, H.: A new structure-based classification of sulfide:quinone oxidoreductases. Proteins: Structure, Function, and Bioinformatics 78 (5), pp. 1073 - 1083 (2010)
Journal Article
Clason, T.; Ruiz, T.; Schägger, H.; Peng, G.; Zickermann, V.; Brandt, U.; Michel, H.; Radermacher, M.: The structure of eukaryotic and prokaryotic complex I. Journal of Structural Biology 169 (1), pp. 81 - 88 (2010)
2009
Journal Article
Baier, J.; Gabrielsen, M.; Oellerich, S.; Michel, H.; van Heel, M.; Cogdell, R. J.; Köhler, J.: Spectral Diffusion and Electron-Phonon Coupling of the B800 BChl a Molecules in LH2 Complexes from Three Different Species of Purple Bacteria. Biophysical Journal 97 (9), pp. 2604 - 2612 (2009)
Journal Article
Koepke, J.; Olkhova, E.; Angerer, H.; Müller, H.; Peng, G.; Michel, H.: High resolution crystal structure of Paracoccus denitrificans cytochrome c oxidase: New insights into the active site and the proton transfer pathways. Structures and Mechanisms in Molecular Bioenergetics, pp. 635 - 645 (2009)
Journal Article
Marcia, M.; Ermler, U.; Peng, G.; Michel, H.: The structure of Aquifex aeolicus sulfide:quinone oxidoreductase, a basis to understand sulfide detoxification and respiration. Proceedings of the National Academy of Sciences of the United States of America 106 (24), pp. 9625 - 9630 (2009)
Journal Article
Olkhova, E.; Kozachkov, L.; Padan, E.; Michel, H.: Combined computational and biochemical study reveals the importance of electrostatic interactions between the ‘‘pH sensor’’ and the cation binding site of the sodium/proton antiporter NhaA of Escherichia coli. Proteins: Structure, Function, and Bioinformatics 76 (3), pp. 548 - 559 (2009)
2008
Journal Article
Dürr, K. L.; Koepke, J.; Hellwig, P.; Müller, H.; Angerer, H.; Peng, G.; Olkhova, E.; Richter, O.-M. H.; Ludwig, B.; Michel, H.: A D-Pathway Mutation Decouples the Paracoccus denitrificans Cytochrome c Oxidase by Altering the Side-Chain Orientation of a Distant Conserved Glutamate. Journal of Molecular Biology 384 (4), pp. 865 - 877 (2008)
Journal Article
Lopez, J. J.; Shukla, A. K.; Reinhart, C.; Schwalbe, H.; Michel, H.; Glaubitz, C.: The Structure of the Neuropeptide Bradykinin Bound to the Human G-Protein Coupled Receptor Bradykinin B2 as Determined by Solid-State NMR Spectroscopy. Angewandte Chemie 120 (9), pp. 1692 - 1695 (2008)
Journal Article
Rimon, A.; Hunte, C.; Michel, H.; Padan, E.: Epitope Mapping of Conformational Monoclonal Antibodies Specific to NhaA Na+/H+ antiporter: Structural and Functional Implications. Journal of Molecular Biology 379 (3), pp. 471 - 481 (2008)
Journal Article
Roy, A.; Shukla, A. K.; Haase, W.; Michel, H.: Employing Rhodobacter sphaeroides to functionally express and purify human G protein-coupled receptors. Biological Chemistry 389, pp. 69 - 78 (2008)
Journal Article
Xia, H.; Liu, L.; Reinhart, C.; Michel, H.: Heterologous expression of human Neuromedin U receptor 1 and its subsequent solubilization and purification. Biochimica et Biophysica Acta - Biomembranes 1778, pp. 2203 - 2209 (2008)
2007
Journal Article
Srinivasan, V.; Netz, D. J. A.; Webert, H.; Mascarenhas, J.; Plerik, A. J.; Michel, H.; Lill, R.: Structure of the Yeast WD40 Domain Protein Cia1, a Component Acting Late in Iron-Sulfur Protein Biogenesis. Structure 15, pp. 1246 - 1257 (2007)
Journal Article
Shukla, A. K.; Haase, W.; Reinhart, C.; Michel, H.: Heterologous expression and characterization of the recombinant bradykinin B2 receptor using the methylotrophic yeast Pichia pastoris. Protein Expression and Purification 55 (1), pp. 1 - 8 (2007)
Journal Article
Olkhova, E.; Padan, E.; Michel, H.: The Influence of Protonation States on the Dynamics of the NhaA Antiporter from Escherichia coli. Biophysical Journal 92, pp. 3784 - 3791 (2007)
Journal Article
Shukla, A. K.; Haase, W.; Reinhart, C.; Michel, H.: Heterologous expression and comparative characterization of the human neuromedin U subtype II receptor using the methylotrophic yeast Pichia pastoris and mammalian cells. The International Journal of Biochemistry & Cell Biology 39 (5), pp. 931 - 942 (2007)
Journal Article
Chillakuri, C. R.; Reinhart, C.; Michel, H.: C-terminal truncated cannabinoid receptor 1 co-expressed with G protein trimer in Sf9 cells exist in a precoupled state and show constitutive activity. FEBS Journal 274, pp. 6106 - 6115 (2007)
Journal Article
Klammt, C.; Srivastava, A.; Eifler, N.; Junge, F.; Beyermann, M.; Schwarz, D.; Michel, H.; Doetsch, V.; Bernhard, F.: Functional analysis of cell-free produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation. FEBS Journal 274, pp. 3257 - 3269 (2007)
Journal Article
Wedemeyer, U.; Peng, G.; Michel, H.; Hartung, K.: Protein AQ_1862 from the Hyperthermophilic Bacterium Aquifex aeolicus is a Porin and Contains Two Conductance Pathways of Different Selectivity. Biophysical Journal 93, pp. 2667 - 2677 (2007)
2006
Journal Article
Shukla, A. K.; Haase, W.; Reinhart, C.; Michel, H.: Functional Overexpression and Characterization of Human Bradykinin Subtype 2 Receptor in Insect Cells Using the Baculovirus System. Journal of Cellular Biochemistry 99 (3), pp. 868 - 877 (2006)
Journal Article
Shukla, A. K.; Haase, W.; Reinhart, C.; Michel, H.: Biochemical and pharmacological characterization of the human bradykinin subtype 2 receptor produced in mammalian cells using the Semliki Forest virus system. Biological Chemistry 387 (5), pp. 569 - 576 (2006)
Journal Article
Farver, O.; Grell, E.; Ludwig, B.; Michel, H.; Pecht, I.: Rates and Equilibrium of CuA to Heme a Electron Transfer in Paracoccus denitrificans Cytochrome c Oxidase. Biophysical Journal 90, pp. 2131 - 2137 (2006)
Journal Article
Olkhova, E.; Hunte, C.; Screpanti, E.; Padan, E.; Michel, H.: Multiconformation continuum electrostatics analysis of the NhaA Na+/H+ antiporter of Escherichia coli with functional implications. Proceedings of the National Academy of Sciences of the United States of America 103 (8), pp. 2629 - 2634 (2006)
Journal Article
André, N.; Cherouati, N.; Prual, C.; Steffan, T.; Zeder-Lutz, G.; Magnin, T.; Pattus, F.; Michel, H.; Wagner, R.; Reinhart, C.: Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Science 15, pp. 1115 - 1126 (2006)
Journal Article
Lundstrom, K.; Wagner, R.; Reinhart, C.; Desmyter, A.; Cherouati, N.; Magnin, T.; Zeder-Lutz, G.; Courtot, M.; Prual, C.; André, N. et al.; Hassaine, G.; Michel, H.; Cambillau, C.; Pattus, F.: Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems. Journal of Structural and Functional Genomics 7, pp. 77 - 91 (2006)
Journal Article
MacMillan, F.; Budiman, K.; Angerer, H.; Michel, H.: The role of tryptophan 272 in the Paracoccus denitrificans cytochrome c oxidase. FEBS Letters 580, pp. 1345 - 1349 (2006)
Journal Article
Peng, G.; Bostina, M.; Radermacher, M.; Rais, I.; Karas, M.; Michel, H.: Biochemical and electron microscopic characterization of the F1F0 ATP Synthase from the hyperthermophilic eubacterium Aquifex aeolicus. FEBS Letters 580, pp. 5934 - 5940 (2006)
Journal Article
Screpanti, E.; Padan, E.; Rimon, A.; Michel, H.; Hunte, C.: Crucial Steps in the Structure Determination of the Na+/H+ Antiporter NhaA in its Native Conformation. Journal of Molecular Biology 362, pp. 192 - 202 (2006)
Journal Article
Shukla, A. K.; Reinhart, C.; Michel, H.: Dimethylsulphoxide as a tool to increase functional expression of heterologously produced GPCRs in mammalian cells. FEBS Letters 580, pp. 4261 - 4265 (2006)
Journal Article
Shukla, A. K.; Reinhart, C.; Michel, H.: Comparative analysis of the human angiotensin II type 1a receptor heterologously produced in insect cells and mammalian cells. Biochemical and Biophysical Research Communications 349, pp. 6 - 14 (2006)
Journal Article
Surade, S.; Klein, M.; Stolt-Bergner, P. C.; Muenke, C.; Roy, A.; Michel, H.: Comparative analysis and "expression space" coverage of the production of prokaryotic membrane proteins for structural genomics. Protein Science 15, pp. 2178 - 2189 (2006)
2005
Journal Article
Barleben, L.; Ma, X.; Koepke, J.; Peng, G.; Michel, H.; Stöckigt, J.: Expression, purification, crystallization and preliminary X-ray analysis of strictosidine glucosidase, an enzyme initiating biosynthetic pathways to a unique diversity of indole alkaloid skeletons. Proteins & Proteomics, pp. 89 - 92 (2005)
Journal Article
Hofmann, C.; Michel, H.; van Heel, M.; Köhler, J.: Multivariate Analysis of Single-Molecule Spectra: Surpassing Spectral Diffusion. Physical Review Letters 94, pp. 195501-1 - 195501-4 (2005)
Journal Article
Hunte, C.; Screpanti, E.; Venturi, M.; Rimon, A.; Padan, E.; Michel, H.: Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH. Nature 435 (7046), pp. 1197 - 1202 (2005)
Journal Article
Olkhova, E.; Helms, V.; Michel, H.: Titration Behavior of Residues at the Entrance of the D-Pathway of Cytochrome c Oxidase from Paracoccus denitrificans Investigated by Continuum Electrostatic Calculations. Biophysical Journal 89, pp. 2324 - 2331 (2005)
Journal Article
Srinivasan, V.; Rajendran, C.; Sousa, F. L.; Melo, A. M.P.; Saraiva, L. M.; Pereira, M. M.; Santana, M.; Teixeira, M.; Michel, H.: Structure at 1.3 Å Resolution of Rhodothermus marinus caa3 Cytochrome c Domain. Journal of Molecular Biology 345, pp. 1047 - 1057 (2005)
2004
Journal Article
Maeda, Y.; Kuroki, R.; Haase, W.; Michel, H.; Reiländer, H.: Comparative analysis of high-affinity ligand binding and G protein coupling of the human CXCR1 chemokine receptor and of a CXCR1-Gi2α fusion protein after heterologous production in baculovirus-infected insect cells. European Journal of Biochemistry 271, pp. 1677 - 1689 (2004)
Journal Article
Olkhova, E.; Hutter, M. C.; Lill, M. A.; Helms, V.; Michel, H.: Dynamic Water Networks in Cytochrome c Oxidase from Paracoccus denitrificans Investigated by Molecular Dynamics Simulations. Biophysical Journal 86 (4), pp. 1873 - 1889 (2004)
Journal Article
Budiman, K.; Kannt, A.; Lyubenova, S.; Richter, O.-M.; Ludwig, B.; Michel, H.; MacMillan, F.: Tyrosine-167: The Origin of the Radical Species Observed in the Reaction of Cytochrome c Oxidase with Hydrogen Peroxide in Paracoccus denitrificans. Biochemistry 43, pp. 11709 - 11716 (2004)
Journal Article
Cismasiu, V. B.; Denes, S. A.; Reiländer, H.; Michel, H.; Szedlacsek, S. E.: The MAM (Meprin/A5-protein/PTPmu) Domain Is a Homophilic Binding Site Promoting the Lateral Dimerization of Receptor-like Protein-tyrosine Phosphatase µ. Journal of Biological Chemistry 279, pp. 26922 - 26931 (2004)
Journal Article
Grünewald, S.; Haase, W.; Molsberger, E.; Michel, H.; Reiländer, H.: Production of the Human D2S Receptor in the Methylotrophic Yeast P. pastoris. Receptors and Channels 10 (1), pp. 37 - 50 (2004)
Journal Article
Hofman, C.; Aartsma, T. J.; Michel, H.; Köhler, J.: Spectral dynamics in the B800 band of LH2 from Rhodospirillum molischianum: a single-molecule study. New Journal of Physics 6, pp. 1 - 15 (2004)
Journal Article
Ma, X.; Koepke, J.; Bayer, A.; Linhard, V.; Fritzsch, G.; Zhang, B.; Michel, H.; Stöckigt, J.: Vinorine synthase from Rauvolfia: the first example of crystallization and preliminary X-ray diffraction analysis of an enzyme of the BAHD superfamily. Biochimica et Biophysica Acta - Proteins and Proteomics 1701 (1-2), pp. 129 - 132 (2004)
Journal Article
Ma, X.; Koepke, J.; Fritzsch, G.; Diem, R.; Kutchan, T. M.; Michel, H.; Stöckigt, J.: Crystallization and preliminary X-ray crystallographic analysis of strictosidine synthase from Rauvolfia: the first member of a novel enzyme family. Biochimica et Biophysica Acta - Proteins and Proteomics 1702, pp. 121 - 124 (2004)
2003
Journal Article
Essen, L.-O.; Harrenga, A.; Ostermeier, C.; Michel: 1.3 Angstrom X-ray structure of an antibody Fv fragment used for induced membrane-protein crystallization. Acta Crystallografica D59, pp. 677 - 687 (2003)
Journal Article
Grimaldi, S.; Ostermann, T.; Weiden, N.; Mogi, T.; Miyoshi, H.; Ludwig, B.; Michel; Prisner, T. F.; MacMillan, F.: Asymmetric binding of the high-affinity QH.- ubisemiquinone in quinol oxidase (bo3) from Escherichia coli studied by multifrequency electron paramagnetic resonance spectroscopy. Biochemistry 42, pp. 5632 - 5639 (2003)
Journal Article
Hofmann, C.; Ketelaars, M.; Matsushita, M.; Michel; Aartsma, T.I.; Koehler, J.: Single-molecule study of the electronic couplings in a circular array of molecules: Light-Harvesting-2-complex from Rhodospirillum molischianum. Physical Review Letters 90, pp. 1 - 4 (2003)
Journal Article
Peng, G.; Fritzsch, G.; Zickermann, V.; Schaegger, H.; Mentele, R.; Lottspeich, F.; Bostina, M.; Radermacher, M.; Huber, R.; Stetter, K. O. et al.; Michel, H.: Isolation, characterization and electron microscopic single particle analysis of the NADH:ubiquinone oxidoreductase (complex I) from the hyperthermophilic eubacterium Aquifex aeolicus. Biochemistry 42, pp. 3032 - 3039 (2003)
Journal Article
Peng, G. H.; Fritzsch; Zickermann, H.; Schaegger, H.; Mentele, R.; Lottspeich, M.; Bostina; Radermacher, M.; Huber, R.; Stetter, K. O. et al.; Michel: Isolation, characterization and electron microscopic single particle analysis of the NADH:ubiquinone oxidoreductase (complex I) from the hyperthermophilic eubacterium Aquifex aeolicus. Biochemistry 42, pp. 3032 - 3039 (2003)
Journal Article
Wendling, M.; Mourik van, F.; Stokkum van, I. H. M.; Salverda, J. M.; Michel, H.; Grondelle van, R.: Low-intensity pump-probe measurements on the B800 band of Rhodospirillum molischianum. Biophysical Journal 84 (1), pp. 440 - 449 (2003)
2002
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Hunte, C.; Michel, H.: Crystallisation of membrane proteins mediated by antibody fragments [Review]. Current Opinion in Structural Biology 12 (4), pp. 503 - 508 (2002)
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Hellwig, P.; Pfitzner, U.; Behr, J.; Rost, B.; Pesavento, R. P.; von Donk, W. v.; Gennis, R. B.; Michel, H.; Ludwig, B.; Mäntele, W.: Vibrational modes of tyrosines in cytochrome c oxidase from Paracoccus denitrificans: FTIR and electrochemical studies on Tyr-D4-labeled and on Tyr280His and Tyr35Phe mutant enzymes. Biochemistry 41 (29), pp. 9116 - 9125 (2002)
Journal Article
Ruitenberg, M.; Kannt, A.; Bamberg, E.; Fendler, K.; Michel, H.: Reduction of cytochrome c oxidase by a second electron leads to proton translocation. Nature 417 (6884), pp. 99 - 102 (2002)
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