Department of Molecular Sociology

Department of Molecular Sociology

Dr. Martin Beck, Director

Research in this group combines biochemical approaches, proteomics and cryo-electron microscopy to study large macromolecular assemblies.

 

Zila, Margiotta, et al. Cell (2021)

Cone-shaped HIV-1 capsids are transported through intact nuclear pores

Zila, Margiotta, et al. Cell (2021)

Turoňová, Sikora, Schürrmann, et al. Science (2020)

In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges

Turoňová, Sikora, Schürrmann, et al. Science (2020)

Allegretti, Zimmerli, et al. Nature (2020)

In cell architecture of the nuclear pore complex and snapshots of its turnover

Allegretti, Zimmerli, et al. Nature (2020)

Turoňová et al., Nature Communications (2020)

Benchmarking tomographic acquisition schemes for high-resolution structural biology

Turoňová et al., Nature Communications (2020)

Bernhard Hampoelz, Andre Schwarz et al. Cell (2019)   > read more

Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis

Bernhard Hampoelz, Andre Schwarz et al. Cell (2019)

> read more

von Appen et al. Nature (2015)   > read more

In situ structural analysis of the human nuclear pore complex

von Appen et al. Nature (2015)

> read more

Romanov et al. Cell (2019)   > read more

Disentangling Genetic and Environmental Effects on the Proteotypes of Individuals

Romanov et al. Cell (2019)

> read more

Becher, Andres-Pons, Romanov, Stein, et al. Cell (2018)   > read more

Pervasive Protein Thermal Stability Variation during the Cell Cycle

Becher, Andres-Pons, Romanov, Stein, et al. Cell (2018)

> read more

Kosinski, Mosalaganti, von Appen, et al. Science (2016) > read more

Molecular architecture of the inner ring scaffold of the human nuclear pore complex

Kosinski, Mosalaganti, von Appen, et al. Science (2016) 

> read more

Mosalaganti, Kosinski, Albert, Schaffer, et al. Nature Communications (2018) > read more

In situ architecture of the algal nuclear pore complex

Mosalaganti, Kosinski, Albert, Schaffer, et al. Nature Communications (2018) 

> read more

Hampoelz et al. Cell (2016)   > read more

Pre-Assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development

Hampoelz et al. Cell (2016)

> read more

Bui, von Appen, et al. Cell (2013)

Integrated structural analysis of the human nuclear pore complex scaffold

Bui, von Appen, et al. Cell (2013)

Research Interests

Functional cellular modules have often been characterised in vitro. However, relatively little is known about their interplay and spatio-temporal arrangement in the context of living cells, i.e. their 'molecular sociology'. We use integrative, in situ structural biology techniques to study the structure, function and assembly of very large macromolecular complexes in their native environment. We rely on a diverse methodological repertoire, including cryo-EM, structural proteomics, biochemistry, imaging, animal model systems and computational modeling (Robinson et al, Nature 2007; Beck and Baumeister, TiCB 2016).  


Projects

 

Selected Publications (Google Scholar Link)

1.
Zila, V., Margiotta, E., Turoňová, B., Müller, T.G., Zimmerli, C.E., Mattei, S., Allegretti, M., Börner, K., Rada, J., Müller, B., Lusic, M., Kräusslich, H.G., Beck, M.
Cone-shaped HIV-1 capsids are transported through intact nuclear pores.
Cell. 2021 Feb 10; 184, 1-15. doi:10.1016/j.cell.2021.01.025.
2.
Allegretti M., Zimmerli C. E., Rantos V., Wilfling F., Ronchi P., Fung H.K.H., Lee CW., Hagen W., Turonova B., Karius K., Börmel M., Zhang X., Müller C.W., Schwab Y., Mahamid J., Pfander B., Kosinski J., Beck M. 
In-cell architecture of the nuclear pore complex and snapshots of its turnover. 
Nature. 2020 Sept 02; 586, 796-800. doi: 10.1038/s41586-020-2670-5.
3.
Turoňová B, Sikora M, Schürmann C, Hagen WJH, Welsch S, Blanc FEC, von Bülow S, Gecht M, Bagola K, Hörner C, v Zandbergen G, Landry J, Doimo de Azevedo NT, Mosalaganti S, Schwarz A, Covino R, Mühlebach MD, Hummer G, Krijnse Locker J, Beck M.
In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges.
Science. 2020 Oct 9; 370, 203-208. doi: 10.1126/science.abd5223.
4.
Hampoelz B., Schwarz A., Ronchi P., Bragulat-Teixidor H., Tischer C., Gaspar I., Ephrussi A., Schwab Y., Beck M.
Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis.
Cell. 2019 Oct 17;179(3):671-686.e17. doi: 10.1016/j.cell.2019.09.022.
5.
Romanov N., Kuhn M., Aebersold R., Ori A., Beck M., Bork P.
Disentangling Genetic and Environmental Effects on the Proteotypes of Individuals.
Cell. 2019 May 16;177(5):1308-1318.e10. doi: 10.1016/j.cell.2019.03.015.
6.
Becher I., Andres-Pons A., Romanov N., Stein F., Schramm M., Baudin F., Helm D., Kurzawa N., Mateus A., Mackmull M.T., Typas A., Mueller C.W., Bork P., Beck M., Savitski M.M.
Pervasive Protein Thermal Stability Variation during the Cell Cycle.
Cell. 2018 May 31;173(6):1495-1507.e18. doi: 10.1016/j.cell.2018.03.053.
7.
Mosalaganti S., Kosinski J., Albert S., Schaffer M., Strenkert D., Salome P.A., Merchant S.S., Plitzko J.M., Baumeister W., Engel B.D., Beck M.
In situ architecture of the algal nuclear pore complex.
Nat Commun. 2018 Jun 18;9(1):2361. doi: 10.1038/s41467-018-04739-y.
8.
Mackmull M.T., Klaus B., Heinze I., Chokkalingam M., Beyer A., Russell R.B., Ori A., Beck M.
Landscape of nuclear transport receptor cargo specificity.
Mol Syst Biol. 2017 Dec 18;13(12):962. doi: 10.15252/msb.20177608.
9.
Hampoelz B., Mackmull M.T., Machado P., Ronchi P., Bui K.H., Schieber N., Santarella-Mellwig R., Necakov A., Andres-Pons A., Philippe J.M., Lecuit T., Schwab Y., Beck M.
Pre-assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development.
Cell. 2016 Jul 28;166(3):664-678. doi: 10.1016/j.cell.2016.06.015.
10.
Ori A., Iskar M., Buczak K., Kastritis P., Parca L., Andres-Pons A., Singer S., Bork P., Beck M.
Spatiotemporal variation of mammalian protein complex stoichiometries.
Genome Biol. 2016 Mar 14;17:47. doi: 10.1186/s13059-016-0912-5.
11.
Kosinski J., Mosalaganti S., von Appen A., Teimer R., DiGuilio A.L., Wan W., Bui K.H., Hagen W.J,, Briggs J.A., Glavy J.S., Hurt E., Beck M.
Molecular architecture of the inner ring scaffold of the human nuclear pore complex.
Science. 2016 Apr 15;352(6283):363-5. doi: 10.1126/science.aaf0643.
12.
von Appen A., Kosinski J., Sparks L., Ori A., DiGuilio A.L., Vollmer B., Mackmull M.T., Banterle N., Parca L., Kastritis P., Buczak K., Mosalaganti S., Hagen W., Andres-Pons A., Lemke E.A., Bork P., Antonin W., Glavy J.S., Bui K.H., Beck M.
In situ structural analysis of the human nuclear pore complex.
Nature. 2015 Oct 1;526(7571):140-143. doi: 10.1038/nature15381.
13.
Ori A., Toyama B.H., Harris M.S., Bock T., Iskar M., Bork P., Ingolia N.T., Hetzer M.W., Beck M.
Integrated Transcriptome and Proteome Analyses Reveal Organ-Specific Proteome Deterioritation in Rats.
Cell Syst 2015, 1(3), 224–237. doi: 10.1016/j.cels.2015.08.012
14.
Bui K.H., von Appen A., DiGuilio A.L., Ori A., Sparks L., Mackmull M.T., Bock T., Hagen W., Andres-Pons A., Glavy J.S., Beck M.
Integrated structural analysis of the human nuclear pore complex scaffold.
Cell. 2013 Dec 5;155(6):1233-43. doi: 10.1016/j.cell.2013.10.055.
15.
Ori A., Banterle N., Iskar M., Andres-Pons A., Escher C., Khanh Bui H., Sparks L., Solis-Mezarino V., Rinner O., Bork P., Lemke E.A., Beck M.
Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines.
Mol Syst Biol. 2013;9:648. doi: 10.1038/msb.2013.4.

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