Department of Molecular Sociology

Department of Molecular Sociology

Dr. Martin Beck, Director

Research in this group combines biochemical approaches, proteomics and cryo-electron microscopy to study large macromolecular assemblies.

 

Research Interests

Functional cellular modules have often been characterised in vitro. However, relatively little is known about their interplay and spatio-temporal arrangement in the context of living cells, i.e. their 'molecular sociology'. We use integrative, in situ structural biology techniques to study the structure, function and assembly of very large macromolecular complexes in their native environment. We rely on a diverse methodological repertoire, including cryo-EM, structural proteomics, biochemistry, imaging, animal model systems and computational modeling (Robinson et al, Nature 2007; Beck and Baumeister, TiCB 2016).  


Projects

 

Selected Publications (Google Scholar Link)

1.
Allegretti M., Zimmerli C. E., Rantos V., Wilfling F., Ronchi P., Fung H.K.H., Lee CW., Hagen W., Turonova B., Karius K., Börmel M., Zhang X., Müller C.W., Schwab Y., Mahamid J., Pfander B., Kosinski J., Beck M. 
In-cell architecture of the nuclear pore complex and snapshots of its turnover. 
Nature. 2020 Sept 02. doi: 10.1038/s41586-020-2670-5.
2.
Turoňová B, Sikora M, Schürmann C, Hagen WJH, Welsch S, Blanc FEC, von Bülow S, Gecht M, Bagola K, Hörner C, v Zandbergen G, Landry J, Doimo de Azevedo NT, Mosalaganti S, Schwarz A, Covino R, Mühlebach MD, Hummer G, Krijnse Locker J, Beck M.
In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges.
Science. 2020 Oct 9; 370, 203-208. doi: 10.1126/science.abd5223.
3.
Hampoelz B., Schwarz A., Ronchi P., Bragulat-Teixidor H., Tischer C., Gaspar I., Ephrussi A., Schwab Y., Beck M.
Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis.
Cell. 2019 Oct 17;179(3):671-686.e17. doi: 10.1016/j.cell.2019.09.022.
4.
Romanov N., Kuhn M., Aebersold R., Ori A., Beck M., Bork P.
Disentangling Genetic and Environmental Effects on the Proteotypes of Individuals.
Cell. 2019 May 16;177(5):1308-1318.e10. doi: 10.1016/j.cell.2019.03.015.
5.
Becher I., Andres-Pons A., Romanov N., Stein F., Schramm M., Baudin F., Helm D., Kurzawa N., Mateus A., Mackmull M.T., Typas A., Mueller C.W., Bork P., Beck M., Savitski M.M.
Pervasive Protein Thermal Stability Variation during the Cell Cycle.
Cell. 2018 May 31;173(6):1495-1507.e18. doi: 10.1016/j.cell.2018.03.053.
6.
Mosalaganti S., Kosinski J., Albert S., Schaffer M., Strenkert D., Salome P.A., Merchant S.S., Plitzko J.M., Baumeister W., Engel B.D., Beck M.
In situ architecture of the algal nuclear pore complex.
Nat Commun. 2018 Jun 18;9(1):2361. doi: 10.1038/s41467-018-04739-y.
7.
Mackmull M.T., Klaus B., Heinze I., Chokkalingam M., Beyer A., Russell R.B., Ori A., Beck M.
Landscape of nuclear transport receptor cargo specificity.
Mol Syst Biol. 2017 Dec 18;13(12):962. doi: 10.15252/msb.20177608.
8.
Hampoelz B., Mackmull M.T., Machado P., Ronchi P., Bui K.H., Schieber N., Santarella-Mellwig R., Necakov A., Andres-Pons A., Philippe J.M., Lecuit T., Schwab Y., Beck M.
Pre-assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development.
Cell. 2016 Jul 28;166(3):664-678. doi: 10.1016/j.cell.2016.06.015.
9.
Ori A., Iskar M., Buczak K., Kastritis P., Parca L., Andres-Pons A., Singer S., Bork P., Beck M.
Spatiotemporal variation of mammalian protein complex stoichiometries.
Genome Biol. 2016 Mar 14;17:47. doi: 10.1186/s13059-016-0912-5.
10.
Kosinski J., Mosalaganti S., von Appen A., Teimer R., DiGuilio A.L., Wan W., Bui K.H., Hagen W.J,, Briggs J.A., Glavy J.S., Hurt E., Beck M.
Molecular architecture of the inner ring scaffold of the human nuclear pore complex.
Science. 2016 Apr 15;352(6283):363-5. doi: 10.1126/science.aaf0643.
11.
von Appen A., Kosinski J., Sparks L., Ori A., DiGuilio A.L., Vollmer B., Mackmull M.T., Banterle N., Parca L., Kastritis P., Buczak K., Mosalaganti S., Hagen W., Andres-Pons A., Lemke E.A., Bork P., Antonin W., Glavy J.S., Bui K.H., Beck M.
In situ structural analysis of the human nuclear pore complex.
Nature. 2015 Oct 1;526(7571):140-143. doi: 10.1038/nature15381.
12.
Ori A., Toyama B.H., Harris M.S., Bock T., Iskar M., Bork P., Ingolia N.T., Hetzer M.W., Beck M.
Integrated Transcriptome and Proteome Analyses Reveal Organ-Specific Proteome Deterioritation in Rats.
Cell Syst 2015, 1(3), 224–237. doi: 10.1016/j.cels.2015.08.012
13.
Bui K.H., von Appen A., DiGuilio A.L., Ori A., Sparks L., Mackmull M.T., Bock T., Hagen W., Andres-Pons A., Glavy J.S., Beck M.
Integrated structural analysis of the human nuclear pore complex scaffold.
Cell. 2013 Dec 5;155(6):1233-43. doi: 10.1016/j.cell.2013.10.055.
14.
Ori A., Banterle N., Iskar M., Andres-Pons A., Escher C., Khanh Bui H., Sparks L., Solis-Mezarino V., Rinner O., Bork P., Lemke E.A., Beck M.
Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines.
Mol Syst Biol. 2013;9:648. doi: 10.1038/msb.2013.4.

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