Martin Beck – Molecular Sociology

Martin Beck – Molecular Sociology

How do molecular modules act in concert to generate complex cellular functions?

 

Translation dynamics in human cells visualized at high resolution reveal cancer drug action

Translation dynamics in human cells visualized at high resolution reveal cancer drug action

Xing, H., Taniguchi, R., Khusainov, I., Kreysing, J.P., et al. Science (2023)

Co-translational binding of importins to nascent proteins

Co-translational binding of importins to nascent proteins

Seidel, M., Romanov, N., Obarska-Kosinska, A., et al. Nature (2023)

AI-based structure prediction empowers integrative structural analysis of human nuclear pores

AI-based structure prediction empowers integrative structural analysis of human nuclear pores

Mosalaganti, S., Obarska-Kosinska, A.,  et al. Science (2022)

Nuclear pores dilate and constrict in cellulo

Nuclear pores dilate and constrict in cellulo

Zimmerli, C. E., Allegretti, M., et al. (Science 2021)
 

Cone-shaped HIV-1 capsids are transported through intact nuclear pores

Cone-shaped HIV-1 capsids are transported through intact nuclear pores

Zila, Margiotta, et al. Cell (2021)

In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges

In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges

Turoňová, Sikora, Schürrmann, et al. Science (2020)

In cell architecture of the nuclear pore complex and snapshots of its turnover

In cell architecture of the nuclear pore complex and snapshots of its turnover

Allegretti, Zimmerli, et al. Nature (2020)

Benchmarking tomographic acquisition schemes for high-resolution structural biology

Benchmarking tomographic acquisition schemes for high-resolution structural biology

Turoňová et al., Nature Communications (2020)

Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis

Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis

Bernhard Hampoelz, Andre Schwarz et al. Cell (2019)

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In situ structural analysis of the human nuclear pore complex

In situ structural analysis of the human nuclear pore complex

von Appen et al. Nature (2015)

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Disentangling Genetic and Environmental Effects on the Proteotypes of Individuals

Disentangling Genetic and Environmental Effects on the Proteotypes of Individuals

Romanov et al. Cell (2019)

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Pervasive Protein Thermal Stability Variation during the Cell Cycle

Pervasive Protein Thermal Stability Variation during the Cell Cycle

Becher, Andres-Pons, Romanov, Stein, et al. Cell (2018)

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Molecular architecture of the inner ring scaffold of the human nuclear pore complex

Molecular architecture of the inner ring scaffold of the human nuclear pore complex

Kosinski, Mosalaganti, von Appen, et al. Science (2016) 

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In situ architecture of the algal nuclear pore complex

In situ architecture of the algal nuclear pore complex

Mosalaganti, Kosinski, Albert, Schaffer, et al. Nature Communications (2018) 

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Pre-Assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development

Pre-Assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development

Hampoelz et al. Cell (2016)

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Integrated structural analysis of the human nuclear pore complex scaffold

Integrated structural analysis of the human nuclear pore complex scaffold

Bui, von Appen, et al. Cell (2013)

Research Interests

Functional cellular modules have often been characterised in vitro. However, relatively little is known about their interplay and spatio-temporal arrangement in the context of living cells, i.e. their 'molecular sociology'. We use integrative, in situ structural biology techniques to study the structure, function and assembly of very large macromolecular complexes in their native environment. We rely on a diverse methodological repertoire, including cryo-EM, structural proteomics, biochemistry, imaging, animal model systems and computational modeling (Robinson et al, Nature 2007; Beck and Baumeister, TiCB 2016).  


Projects

 

Selected Publications (Google Scholar Link)

Mosalaganti, S.; Obarska-Kosinska, A.; Siggel, M.; Taniguchi, R.; Turoňová, B.; Zimmerli, C. E.; Buczak, K.; Schmidt, F. H.; Margiotta, E.; Mackmull, M.-T. et al.
AI-based structure prediction empowers integrative structural analysis of human nuclear pores.
Science. 2022 June; 376 (6598), doi: 10.1126/science.abm9506.
Zimmerli C. E., Allegretti M., Rantos V., Goetz S. K., Obarska-Kosinska A., Zagoriy I., Halavatyi A., Hummer G., Mahamid J., Kosinski J., Beck M.
Nuclear pores dilate and constrict in cellulo.
Science. 2021 Nov; 374 (6573). doi: 10.1126/science.abd9776.
Zila, V., Margiotta, E., Turoňová, B., Müller, T.G., Zimmerli, C.E., Mattei, S., Allegretti, M., Börner, K., Rada, J., Müller, B., Lusic, M., Kräusslich, H.G., Beck, M.
Cone-shaped HIV-1 capsids are transported through intact nuclear pores.
Cell. 2021 Feb; 184, 1-15. doi:10.1016/j.cell.2021.01.025.
Allegretti M., Zimmerli C. E., Rantos V., Wilfling F., Ronchi P., Fung H.K.H., Lee CW., Hagen W., Turonova B., Karius K., Börmel M., Zhang X., Müller C.W., Schwab Y., Mahamid J., Pfander B., Kosinski J., Beck M. 
In-cell architecture of the nuclear pore complex and snapshots of its turnover. 
Nature. 2020 Sept; 586, 796-800. doi: 10.1038/s41586-020-2670-5.
Turoňová B, Sikora M, Schürmann C, Hagen WJH, Welsch S, Blanc FEC, von Bülow S, Gecht M, Bagola K, Hörner C, v Zandbergen G, Landry J, Doimo de Azevedo NT, Mosalaganti S, Schwarz A, Covino R, Mühlebach MD, Hummer G, Krijnse Locker J, Beck M.
In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges.
Science. 2020 Oct; 370, 203-208. doi: 10.1126/science.abd5223.
Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis.
Cell. 2019 Oct; 179(3):671-686.e17. doi: 10.1016/j.cell.2019.09.022.
Romanov N., Kuhn M., Aebersold R., Ori A., Beck M., Bork P.
Disentangling Genetic and Environmental Effects on the Proteotypes of Individuals.
Cell. 2019 May; 177(5):1308-1318.e10. doi: 10.1016/j.cell.2019.03.015.
Becher I., Andres-Pons A., Romanov N., Stein F., Schramm M., Baudin F., Helm D., Kurzawa N., Mateus A., Mackmull M.T., Typas A., Mueller C.W., Bork P., Beck M., Savitski M.M.
Pervasive Protein Thermal Stability Variation during the Cell Cycle.
Cell. 2018 May; 173(6):1495-1507.e18. doi: 10.1016/j.cell.2018.03.053.
Mosalaganti S., Kosinski J., Albert S., Schaffer M., Strenkert D., Salome P.A., Merchant S.S., Plitzko J.M., Baumeister W., Engel B.D., Beck M.
In situ architecture of the algal nuclear pore complex.
Nat Commun. 2018 Jun; 9(1):2361. doi: 10.1038/s41467-018-04739-y.
Mackmull M.T., Klaus B., Heinze I., Chokkalingam M., Beyer A., Russell R.B., Ori A., Beck M.
Landscape of nuclear transport receptor cargo specificity.
Mol Syst Biol. 2017 Dec; 13(12):962. doi: 10.15252/msb.20177608.
Hampoelz B., Mackmull M.T., Machado P., Ronchi P., Bui K.H., Schieber N., Santarella-Mellwig R., Necakov A., Andres-Pons A., Philippe J.M., Lecuit T., Schwab Y., Beck M.
Pre-assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development.
Cell. 2016 Jul; 166(3):664-678. doi: 10.1016/j.cell.2016.06.015.
Ori A., Iskar M., Buczak K., Kastritis P., Parca L., Andres-Pons A., Singer S., Bork P., Beck M.
Spatiotemporal variation of mammalian protein complex stoichiometries.
Genome Biol. 2016 Mar 14;17:47. doi: 10.1186/s13059-016-0912-5.
Kosinski J., Mosalaganti S., von Appen A., Teimer R., DiGuilio A.L., Wan W., Bui K.H., Hagen W.J,, Briggs J.A., Glavy J.S., Hurt E., Beck M.
Molecular architecture of the inner ring scaffold of the human nuclear pore complex.
Science. 2016 Apr 15;352(6283):363-5. doi: 10.1126/science.aaf0643.
von Appen A., Kosinski J., Sparks L., Ori A., DiGuilio A.L., Vollmer B., Mackmull M.T., Banterle N., Parca L., Kastritis P., Buczak K., Mosalaganti S., Hagen W., Andres-Pons A., Lemke E.A., Bork P., Antonin W., Glavy J.S., Bui K.H., Beck M.
In situ structural analysis of the human nuclear pore complex.
Nature. 2015 Oct 1;526(7571):140-143. doi: 10.1038/nature15381.
Ori A., Toyama B.H., Harris M.S., Bock T., Iskar M., Bork P., Ingolia N.T., Hetzer M.W., Beck M.
Integrated Transcriptome and Proteome Analyses Reveal Organ-Specific Proteome Deterioritation in Rats.
Cell Syst 2015, 1(3), 224–237. doi: 10.1016/j.cels.2015.08.012
Bui K.H., von Appen A., DiGuilio A.L., Ori A., Sparks L., Mackmull M.T., Bock T., Hagen W., Andres-Pons A., Glavy J.S., Beck M.
Integrated structural analysis of the human nuclear pore complex scaffold.
Cell. 2013 Dec 5;155(6):1233-43. doi: 10.1016/j.cell.2013.10.055.
Ori A., Banterle N., Iskar M., Andres-Pons A., Escher C., Khanh Bui H., Sparks L., Solis-Mezarino V., Rinner O., Bork P., Lemke E.A., Beck M.
Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines.
Mol Syst Biol. 2013;9:648. doi: 10.1038/msb.2013.4.

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