Florian Wilfling Publications

2025

Journal Article

Licheva, M.; Babic, R.; Pflaum, J.; Mancilla, H.; Wilfling, F.; Kraft, C.: To degrade or not to degrade: how phase separation modulates selective autophagy. Autophagy (2025)

MPG.PuRe

DOI

Full

Journal Article

Licheva, M.; Pflaum, J.; Babic, R.; Mancilla, H.; Elsässer, J.; Boyle, E.; Hollenstein, D. M.; Jimenez-Niebla, J.; Pleyer, J.; Heinrich, M. et al.; Wieland, F.-G.; Brenneisen, J.; Eickhorst, C.; Brenner, J.; Jiang, S.; Hartl, M.; Welsch, S.; Hunte, C.; Timmer, J.; Wilfling, F.; Kraft, C.: Phase separation of initiation hubs on cargo is a trigger switch for selective autophagy. Nature Cell Biology 27 (2), pp. 283 - 297 (2025)

MPG.PuRe

DOI

Full

Journal Article

Kraus, F.; He, Y.; Swarup, S.; Overmyer, K. A.; Jiang, Y.; Brenner, J.; Capitanio, C.; Bieber, A.; Jen, A.; Nightingale, N. M. et al.; Anderson, B. J.; Lee, C.; Paulo, J. A.; Smith, I. R.; Plitzko, J. M.; Gygi, S. P.; Schulman, B. A.; Wilfling, F.; Coon, J. J.; Harper, J. W.: Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST. Science Advances 11 (4), eadu5787 (2025)

MPG.PuRe

DOI

Full

2024

Journal Article

Grizel, A. V.; Gorsheneva, N. A.; Stevenson, J. B.; Pflaum, J.; Wilfling, F.; Rubel, A. A.; Chernoff, Y. O.: Osmotic stress induces formation of both liquid condensates and amyloids by a yeast prion domain. Journal of Biological Chemistry 300 (10), 107766 (2024)

MPG.PuRe

DOI

Full

Journal Article

Lizarrondo, J.; Wilfling, F.: Selective autophagy of macromolecular complexes: what does it take to be taken? Journal of Molecular Biology 436, 168574 (2024)

MPG.PuRe

DOI

Full

Journal Article

Wilfling, F.; Graef, M.: Molecular Mechanisms and Physiological Functions of Autophagy. Journal of Molecular Biology 436, 168692 (2024)

MPG.PuRe

DOI

Journal Article

Zhao, D. Y.; Bäuerlein, F. J. B.; Saha, I.; Hartl, F. U.; Baumeister, W.; Wilfling, F.: Autophagy preferentially degrades non-fibrillar polyQ aggregates. Molecular Cell 84 (10), pp. 1980 - 1994.e8 (2024)

MPG.PuRe

DOI

Full

Journal Article

Hoyer, M. J.; Capitanio, C.; Smith, I. R.; Paoli, J. C.; Bieber, A.; Jiang, Y.; Paulo, J. A.; Gonzalez-Lozano, M. A.; Baumeister, W.; Wilfling, F. et al.; Schulman, B. A.; Harper, J. W.: Combinatorial selective ER-phagy remodels the ER during neurogenesis. Nature Cell Biology 26 (3), pp. 378 - 392 (2024)

MPG.PuRe

DOI

Full

2023

Journal Article

Wilfling, F.; Kaksonen, M.; Stachowiak, J.: Protein condensates as flexible platforms for membrane traffic. Current Opinion in Cell Biology 85, 102258 (2023)

MPG.PuRe

DOI

Journal Article

Boyle, E.; Wilfling, F.: Autophagy as a caretaker of nuclear integrity. FEBS Letters 597, pp. 2728 - 2738 (2023)

MPG.PuRe

DOI

Full

Journal Article

Brenner, J.; Klumpe, S.; Wilfling, F.; Plitzko, J. M.: Towards Temporal Resolution in Correlative Cryo-electron Tomography. Microscopy and Microanalysis 29 (Supplement\1), pp. 1937 - 1938 (2023)

MPG.PuRe

DOI

Journal Article

Schmitt, D.; Bozkurt, S.; Henning-Domres, P.; Huesmann, H.; Eimer, S.; Bindila, L.; Behrends, C.; Boyle, E.; Wilfling, F.; Tascher, G. et al.; Münch, C.; Behl, C.; Kern, A.: FACS-mediated isolation of native autophagic vesicles. Autophagy 19 (7), pp. 2146 - 2147 (2023)

MPG.PuRe

DOI

Full

Journal Article

Capitanio, C.; Bieber, A.; Wilfling, F.: How Membrane Contact Sites Shape the Phagophore. Contact 6 (2023)

MPG.PuRe

DOI

Full

Journal Article

Li, M.; Tripathi-Giesgen, I.; Schulman, B. A.; Baumeister, W.; Wilfling, F.: In situ snapshots along a mammalian selective autophagy pathway. Proceedings of the National Academy of Sciences of the United States of America 120 (12), e2221712120 (2023)

MPG.PuRe

DOI

Full

2022

Journal Article

Schmitt, D.; Bozkurt, S.; Henning-Domres, P.; Huesmann, H.; Eimer, S.; Bindila, L.; Behrends, C.; Boyle, E.; Wilfling, F.; Tascher, G. et al.; Münch, C.; Behl, C.; Kern, A.: Lipid and protein content profiling of isolated native autophagic vesicles. EMBO Reports, e53065 (2022)

MPG.PuRe

DOI

Full

Journal Article

Bieber, A.; Capitanio, C.; Erdmann, P. S.; Fiedler, F.; Beck, F.; Lee, C.-W.; Li, D.; Hummer, G.; Schulman, B. A.; Baumeister, W. et al.; Wilfling, F.: In situ structural analysis reveals membrane shape transitions during autophagosome formation. Proceedings of the National Academy of Sciences of the United States of America 119 (39), e2209823119 (2022)

MPG.PuRe

DOI

Full

Journal Article

Enenkel, C.; Kang, R. W.; Wilfling, F.; Ernst, O. P.: Intracellular localization of the proteasome in response to stress conditions. The Journal of Biological Chemistry 298 (7), 102083 (2022)

MPG.PuRe

DOI

Full

Journal Article

Qiao, S.; Lee, C.-W.; Sherpa, D.; Chrustowicz, J.; Cheng, J.; Duennebacke, M.; Steigenberger, B.; Karayel, O.; Vu, D. T.; von Gronau, S. et al.; Mann, M.; Wilfling, F.; Schulman, B. A.: Cryo-EM structures of Gid12-bound GID E3 reveal steric blockade as a mechanism inhibiting substrate ubiquitylation. Nature Communications 13, 3041 (2022)

MPG.PuRe

DOI

Journal Article

Boyle, E.; Wilfling, F.: Bypassing the nuclear gate: A non-canonical entry pathway for the mitochondrial pyruvate dehydrogenase complex. Molecular Cell 82 (5), pp. 886 - 888 (2022)

MPG.PuRe

DOI

2021

Journal Article

Bieber, A.; Capitanio, C.; Wilfling, F.; Plitzko, J.; Erdmann, P. S.: Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography. Journal of Visualized Experiments (176), e62886 (2021)

MPG.PuRe

DOI

Full

Journal Article

Wilfling, F.; Lee, C.-W.; Erdmann, P. S.; Baumeister, W.: Autophagy ENDing unproductive phase-separated endocytic protein deposits. Autophagy 17 (10), pp. 3264 - 3265 (2021)

MPG.PuRe

DOI

Journal Article

Erdbrügger, P.; Wilfling, F.: p62 condensates are a hub for proteasome-mediated protein turnover in the nucleus. Proceedings of the National Academy of Sciences of the United States of America 118 (37), e2113647118 (2021)

MPG.PuRe

DOI

Journal Article

Erdmann, P. S.; Hou, Z.; Klumpe, S.; Khavnekar, S.; Beck, F.; Wilfling, F.; Plitzko, J. M.; Baumeister, W.: In situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli. Nature Communications 12 (1), 5364 (2021)

MPG.PuRe

DOI

2020

Journal Article

Wilfling, F.; Lee, C.-W.; Erdmann, P. S.; Zheng, Y.; Sherpa, D.; Jentsch, S.; Pfander, B.; Schulman, B. A.; Baumeister, W.: A Selective Autophagy Pathway for Phase-Separated Endocytic Protein Deposits. Molecular Cell 80 (5), pp. 764 - 778 (2020)

MPG.PuRe

DOI

Journal Article

Bittmann, J.; Grigaitis, R.; Galanti, L.; Amarell, S.; Wilfling, F.; Matos, J.; Pfander, B.: An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases. eLife 9, e52459 (2020)

MPG.PuRe

DOI

Journal Article

Lee, C.-W.; Wilfling, F.; Ronchi, P.; Allegretti, M.; Mosalaganti, S.; Jentsch, S.; Beck, M.; Pfander, B.: Selective autophagy degrades nuclear pore complexes. Nature Cell Biology 22 (2), pp. 159 - 166 (2020)

MPG.PuRe

DOI

2015

Journal Article

Frank, D. O.; Dengjel, J.; Wilfling, F.; Kozjak-Pavlovic, V.; Häcker, G.; Weber, A.: The Pro-Apoptotic BH3-Only Protein Bim Interacts with Components of the Translocase of the Outer Mitochondrial Membrane (TOM). PLoS One 10 (4), e0123341 (2015)

MPG.PuRe

DOI

2014

Journal Article

Wilfling, F.; Haas, J. T.; Walther, T. C.; Farese, R. V.: Lipid droplet biogenesis. Current Opinion in Cell Biology 29, pp. 39 - 45 (2014)

MPG.PuRe

DOI

Journal Article

Wilfling, F.; Thiam, A. R.; Olarte, M.-J.; Wang, J.; Beck, R.; Gould, T. J.; Allgeyer, E. S.; Pincet, F.; Bewersdorf, J.; Farese, R. V. et al.; Walther, T. C.: Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targeting. eLife 3, e01607 (2014)

MPG.PuRe

DOI

2013

Journal Article

Thiam, A. R.; Antonny, B.; Wang, J.; Delacotte, J.; Wilfling, F.; Walther, T. C.; Beck, R.; Rothman, J. E.; Pincet, F.: COPI buds 60-nm lipid droplets from reconstituted water-phospholipid-triacylglyceride interfaces, suggesting a tension clamp function. Proceedings of the National Academy of Sciences of the United States of America 110 (33), pp. 13244 - 13249 (2013)

MPG.PuRe

DOI

Journal Article

Mejhert, N.; Wilfling, F.; Esteve, D.; Galitzky, J.; Pellegrinelli, V.; Kolditz, C.-I.; Viguerie, N.; Tordjman, J.; Näslund, E.; Trayhurn, P. et al.; Lacasa, D.; Dahlman, I.; Stich, V.; Lång, P.; Langin, D.; Bouloumié, A.; Clément, K.; Rydén, M.: Semaphorin 3C is a novel adipokine linked to extracellular matrix composition. Diabetologia 56 (8), pp. 1792 - 1801 (2013)

MPG.PuRe

DOI

Journal Article

Krahmer, N.; Hilger, M.; Kory, N.; Wilfling, F.; Stoehr, G.; Mann, M.; Farese Jr., R. V.; Walther, T. C.: Protein Correlation Profiles Identify Lipid Droplet Proteins with High Confidence. Molecular and Cellular Proteomics 12 (5), pp. 1115 - 1126 (2013)

MPG.PuRe

DOI

Journal Article

Wilfling, F.; Wang, H.; Haas, J. T.; Krahmer, N.; Gould, T. J.; Uchida, A.; Cheng, J.-X.; Graham, M.; Christiano, R.; Fröhlich, F. et al.; Liu, X.; Buhman, K. K.; Coleman, R. A.; Bewersdorf, J.; Farese, R. V.; Walther, T. C.: Triacylglycerol synthesis enzymes mediate lipid droplet growth by relocalizing from the ER to lipid droplets. Developmental Cell 24 (4), pp. 384 - 399 (2013)

MPG.PuRe

DOI

2012

Journal Article

Wilfling, F.; Weber, A.; Potthoff, S.; Vögtle, F.-N.; Meisinger, C.; Paschen, S. A.; Häcker, G.: BH3-only proteins are tail-anchored in the outer mitochondrial membrane and can initiate the activation of Bax. Cell Death and Differentiation 19 (8), pp. 1328 - 1336 (2012)

MPG.PuRe

DOI

Full

2011

Journal Article

Krahmer, N.; Guo, Y.; Wilfling, F.; Hilger, M.; Lingrell, S.; Heger, K.; Newman, H. W.; Schmidt-Supprian, M.; Vance, D. E.; Mann, M. et al.; Farese, R. V.; Walther, T. C.: Phosphatidylcholine synthesis for lipid droplet expansion is mediated by localized activation of CTP:phosphocholine cytidylyltransferase. Cell Metabolism 14 (4), pp. 504 - 515 (2011)

MPG.PuRe

DOI

2007

Journal Article

Weber, A.; Paschen, S. A.; Heger, K.; Wilfling, F.; Frankenberg, T.; Bauerschmitt, H.; Seiffert, B. M.; Kirschnek, S.; Wagner, H.; Häcker, G.: Bim_s-induced apoptosis requires mitochondrial localization but not interaction with anti-apoptotic Bcl-2 proteins. The Journal of Cell Biology: JCB 177 (4), pp. 625 - 636 (2007)

MPG.PuRe

DOI

Florian Wilfling Publications

Journal Article (36)