DAAD - Program for scientific staff exchange between Germany and Portugal

Elucidating the 3D structures of membrane protein complexes in sulfur metabolism

This project aims to use cryo-electron microscopy (cryo-EM) for the structural characterization of two vital membrane complexes involved in the anaerobic respiration of sulfur-metabolizing bacteria: the DsrMKJOP and QrcABCD membrane protein complexes from Archaeoglobus fulgidus and Desulfovibrio vulgaris Hildenborough, respectively. These complexes are widespread and essential for sulfur compound respiration, yet their structure and function remain unknown. Determining their structure will be a significant breakthrough in understanding the bioenergetics of anaerobic metabolism in these organisms, with important health and environmental implications.

The goal is to strengthen and maintain our collaboration with the laboratories led by Inês Pereira and Margarida Archer. This presents a valuable opportunity for team members to learn about cultivating sulfur-metabolizing organisms, purifying membrane complexes from native sources, and exploring the bioenergetics and microbial physiology of this important group of organisms.