Integrative Structural Biology

Department of Molecular Sociology (MS)

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Studying architecture of complex macromolecular assemblies in their native context requires the integration of traditional in vitro structural biology methods such as X-ray crystallography and single particle electron microscopy with approaches that facilitate data integration and structural analysis in situ, such as cryo electron tomography, subtomogram averaging and cross-linking mass spectrometry. We are continuously developing novel methods towards this.

Selected Publications

Mosalaganti, S.; Obarska-Kosinska, A.; Siggel, M.; Taniguchi, R.; Turoňová, B.; Zimmerli, C. E.; Buczak, K.; Schmidt, F. H.; Margiotta, E.; Mackmull, M.-T. et al.

AI-based structure prediction empowers integrative structural analysis of human nuclear pores.

Science. 2022 June; 376 (6598). doi: 10.1126/science.abm9506.

DOI

Zimmerli, C. E.; Allegretti, M.; Rantos, V.; Goetz, S. K.; Obarska-Kosinska, A.; Zagoriy, I.; Halavatyi, A.; Hummer, G.; Mahamid, J.; Kosinski, J.; Beck, M., et al.

Nuclear pores dilate and constrict in cellulo.

Science. 2021 Nov; 374(6573). doi:10.1126/science.abd9776.

DOI

Turonova B, Hagen WJ, Obr M, Kräusslich HG, Beck M.

Benchmarking tomographic acquisition schemes for high-resolution structural biology.

Nature Communications, 2020. doi: 10.1038/s41467-020-14535-2.

DOI

Kosinski J, Mosalaganti S, von Appen A, Teimer R, DiGuilio AL, Wan W, Bui KH, Hagen WJ, Briggs JA, Glavy JS, Hurt E, Beck M.

Molecular architecture of the inner ring scaffold of the human nuclear pore complex.

Science. 2016 Apr; 352(6283):363-5. doi: 10.1126/science.aaf0643.

DOI

von Appen A, Kosinski J, Sparks L, Ori A, DiGuilio AL, Vollmer B, Mackmull MT, Banterle N, Parca L, Kastritis P, Buczak K, Mosalaganti S, Hagen W, Andres-Pons A, Lemke EA, Bork P, Antonin W, Glavy JS, Bui KH, Beck M.

In situ structural analysis of the human nuclear pore complex.

Nature. 2015 Oct; 526(7571):140-143. doi: 10.1038/nature15381.

DOI

Kosinski J, von Appen A, Ori A, Karius K, Müller CW, Beck M.

Xlink Analyzer: software for analysis and visualization of cross-linking data in the context of three-dimensional structures.

J Struct Biol. 2015 Mar;189(3):177-83. doi: 10.1016/j.jsb.2015.01.014.

DOI